NDLI: Development of multiplex real-time hybridization probe reverse transcriptase polymerase chain reaction for specific detection and differentiation of Enterovirus 71 and Coxsackievirus A16.

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Development of multiplex real-time hybridization probe reverse transcriptase polymerase chain reaction for specific detection and differentiation of Enterovirus 71 and Coxsackievirus A16.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Tan, Eng Lee Chow, Vincent Tak Kwong Quak, Seng Hock Yeo, Wei Cheng Andrea Poh, Chit Laa
Description	Country affiliation: Singapore Author Affiliation: Tan EL ( Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.)
Abstract	Large outbreaks of hand, foot, and mouth disease have been reported in the Asia Pacific region over the last few years and resulted in significant fatalities. The 2 main etiologic agents are Enterovirus 71 (EV71) and Coxsackievirus A16 (CA16). Both viruses are closely related genetically and show similar clinical symptoms. However, EV71 are associated with neurologic complications and can lead to fatalities. In this study, we developed a multiplex real-time hybridization probe reverse transcriptase polymerase chain reaction to detect and differentiate EV71 from CA16 using the LightCycler (Roche Molecular Biochemicals). Specific primers and hybridization probes were designed based on highly conserved VP1 region of EV71 or CA16. Our results showed high specificity and sensitivities in detecting EV71 or CA16 from 67 clinical specimens, and no other enterovirus serotype was detected. Rapid diagnosis to differentiate EV71 from CA16 in outbreak situations will enable pediatricians to identify and manage the patients more effectively.
File Format	HTM / HTML
ISSN	07328893
Issue Number	3
Volume Number	61
e-ISSN	18790070
Journal	Diagnostic Microbiology and Infectious Disease
Language	English
Publisher	Elsevier
Publisher Date	2008-07-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Microbiology Discipline Communicable Diseases Coxsackievirus Infections Diagnosis Enterovirus Infections Enterovirus Classification Isolation & Purification Nucleic Acid Hybridization Methods Reverse Transcriptase Polymerase Chain Reaction Virology Genetics Genes, Viral Humans Oligonucleotide Probes Sensitivity And Specificity Evaluation Studies Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Microbiology (medical)

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