| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
May, Eugenie Wong Soon Lin, Szu-Ting Lin, Chi-Chen Chang, Jo-Fan Hung, Eric Lo, Yi-Wen Lin, Li-Hsun Hu, Ren-Yu Feng, Chi-Lun Lin, Dai-Ying Wu, Shine-Bei Lee, Wen-Chi Lyu, Kevin W. Chou, Hsiu-Chuan Chan, Hong-Lin |
| Description |
Country affiliation: Taiwan Author Affiliation: May EW ( Institute of Bioinformatics and Structural Biology and Department of Medical Sciences, National Tsing Hua University, Hsinchu, Taiwan.); Lin ST ( Institute of Bioinformatics and Structural Biology and Department of Medical Sciences, National Tsing Hua University, Hsinchu, Taiwan.); Lin CC ( Institute of Biomedical Science, National Chung-Hsing University, Taichung, Taiwan); Chang JF ( Institute of Bioinformatics and Structural Biology and Department of Medical Sciences, National Tsing Hua University, Hsinchu, Taiwan.); Hung E ( Department of Applied Science, National Hsinchu University of Education, Hsinchu, Taiwan.); Lo YW ( Department of Applied Science, National Hsinchu University of Education, Hsinchu, Taiwan.); Lin LH ( Institute of Bioinformatics and Structural Biology and Department of Medical Sciences, National Tsing Hua University, Hsinchu, Taiwan.); Hu RY ( Institute of Bioinformatics and Structural Biology and Department of Medical Sciences, National Tsing Hua University, Hsinchu, Taiwan.); Feng CL ( Department of Applied Science, National Hsinchu University of Education, Hsinchu, Taiwan.); Lin DY ( Institute of Bioinformatics and Structural Biology and Department of Medical Sciences, National Tsing Hua University, Hsinchu, Taiwan.); Wu SB ( Department of Applied Science, National Hsinchu University of Education, Hsinchu, Taiwan.); Lee WC ( Department of Applied Science, National Hsinchu University of Education, Hsinchu, Taiwan.); Lyu KW ( Lutheran Medical Center, Brooklyn, NY, USA); Chou HC ( Department of Applied Science, National Hsinchu University of Education, Hsinchu, Taiwan. Electronic address: hlchan@life.nthu.edu.tw.); Chan HL ( Institute of Bioinformatics and Structural Biology and Department of Medical Sciences, National Tsing Hua University, Hsinchu, Taiwan. Electronic address: chouhc@mail.nhcue.edu.tw.) |
| Abstract |
Drug resistance is a frequent cause of failure in cancer chemotherapy treatments. In this study, a pair of uterine sarcoma cancer lines, MES-SA, and doxorubicin-resistant partners, MES-SA/DxR-2µM cells and MES-SA/DxR-8µM cells, as a model system to investigate resistance-dependent proteome alterations and to identify potential therapeutic targets. We used two-dimensional differential gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) to perform this research and the results revealed that doxorubicin-resistance altered the expression of 208 proteins in which 129 identified proteins showed dose-dependent manners in response to the levels of resistance. Further studies have used RNA interference, H2A.X phosphorylation assay, cell viability analysis, and analysis of apoptosis against reticulocalbin-1 (RCN1) proteins, to prove its potency on the formation of doxorubicin resistance as well as the attenuation of doxorubicin-associated DNA double strand breakage. To sum up, our results provide useful diagnostic markers and therapeutic candidates such as RCN1 for the treatment of doxorubicin-resistant uterine cancer. |
| File Format |
HTM / HTML |
| ISSN |
10436618 |
| Volume Number |
90 |
| e-ISSN |
10961186 |
| Journal |
Pharmacological Research |
| Language |
English |
| Publisher |
Elsevier |
| Publisher Date |
2014-12-01 |
| Publisher Place |
Netherlands |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Discipline Pharmacology Antibiotics, Antineoplastic Pharmacology Calcium-binding Proteins Metabolism Doxorubicin Drug Resistance, Neoplasm Physiology Uterine Neoplasms Apoptosis Drug Effects Genetics Cell Line, Tumor Down-regulation Electrophoresis, Gel, Two-dimensional Female Humans Proteome Rna, Small Interfering Administration & Dosage Spectrometry, Mass, Matrix-assisted Laser Desorption-ionization Up-regulation Journal Article Research Support, Non-u.s. Gov't |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Pharmacology |
