NDLI: Proteome analysis of round-headed and normal spermatozoa by 2-D fluorescence difference gel electrophoresis and mass spectrometry.

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Proteome analysis of round-headed and normal spermatozoa by 2-D fluorescence difference gel electrophoresis and mass spectrometry.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Liao, Ting-Ting Xiang, Zhen Zhu, Wen-Bing Fan, Li-Qing
Description	Country affiliation: China Author Affiliation: Liao TT ( Institute of Human Reproduction and Stem Cell Engineering, Central South University, Changsha 410078, China.)
Abstract	Globozoospermia is a severe form of teratozoospermia characterized by round-headed spermatozoa with an absent acrosome, an aberrant nuclear membrane and midpiece defects. Globozoospermia is diagnosed by the presence of 100% round-headed spermatozoa on semen analysis, and patients with this condition are absolutely infertile. The objective of this study was to investigate the differences in protein expression between human round-headed and normal spermatozoa. Two-dimensional (2-D) fluorescence difference gel electrophoresis (DIGE) coupled with mass spectrometry (MS) was used in this study. Over 61 protein spots were analysed in each paired normal/round-headed comparison, using DIGE technology along with an internal standard. In total, 35 protein spots identified by tandem mass spectrometry (MS/MS) exhibited significant changes (paired t-test, P < 0.05) in the expression level between normal and round-headed spermatozoa. A total of nine proteins were found to be upregulated and 26 proteins were found to be downregulated in round-headed spermatozoa compared with normal spermatozoa. The differentially expressed proteins that we identified may have important roles in a variety of cellular processes and structures, including spermatogenesis, cell skeleton, metabolism and spermatozoa motility.
File Format	HTM / HTML
ISSN	1008682X
e-ISSN	17457262
DOI	10.1038/aja.2009.59
Journal	Asian Journal of Andrology
Issue Number	6
Volume Number	11
Language	English
Publisher	Wolters Kluwer - Medknow
Publisher Date	2009-11-01
Publisher Place	China
Access Restriction	Open
Subject Keyword	Discipline Urology Infertility, Male Genetics Proteome Spermatozoa Abnormalities Down-regulation Electrophoresis, Gel, Two-dimensional Proteomics Spectrometry, Mass, Matrix-assisted Laser Desorption-ionization Sperm Head Ultrastructure Metabolism Tandem Mass Spectrometry Up-regulation Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Urology

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