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The proteomic investigation of chromatin functional domains reveals novel synergisms among distinct heterochromatin components.

Year: 2013 Volume: 12 Issue: 2

Year: 2013 Volume: 12 Issue: 1

Year: 2012 Volume: 11

Year: 2011 Volume: 10

The proteomic investigation of chromatin functional domains reveals novel synergisms among distinct heterochromatin components.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Soldi, Monica Bonaldi, Tiziana
Description	Country affiliation: Italy Author Affiliation: Soldi M ( Department of Experimental Oncology, European Institute of Oncology (IEO), Via Adamello 16, Milan, Italy.)
Abstract	Chromatin is a highly dynamic, well-structured nucleoprotein complex of DNA and proteins that controls virtually all DNA transactions. Chromatin dynamicity is regulated at specific loci by the presence of various associated proteins, histones, post-translational modifications, histone variants, and DNA methylation. Until now the characterization of the proteomic component of chromatin domains has been held back by the challenge of enriching distinguishable, homogeneous regions for subsequent mass spectrometry analysis. Here we describe a modified protocol for chromatin immunoprecipitation combined with quantitative proteomics based on stable isotope labeling by amino acids in cell culture to identify known and novel histone modifications, variants, and complexes that specifically associate with silent and active chromatin domains. Our chromatin proteomics strategy revealed unique functional interactions among various chromatin modifiers, suggesting new regulatory pathways, such as a heterochromatin-specific modulation of DNA damage response involving H2A.X and WICH, both enriched in silent domains. Chromatin proteomics expands the arsenal of tools for deciphering how all the distinct protein components act together to enforce a given region-specific chromatin status.
File Format	HTM / HTML
ISSN	15359476
e-ISSN	15359484
DOI	10.1074/mcp.M112.024307
Journal	Molecular & Cellular Proteomics
Issue Number	3
Volume Number	12
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2013-03-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Proteomics Chromatin Metabolism Heterochromatin Histones Proteome Proteomics Adenosine Triphosphatases Genetics Animals Blotting, Western Chromatin Immunoprecipitation Dna Damage Hela Cells Isotope Labeling Lysine Methylation Mice Nih 3t3 Cells Phosphorylation Protein Processing, Post-translational Tandem Mass Spectrometry Transcription Factors Tyrosine Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine Analytical Chemistry Molecular Biology Biochemistry

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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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The Proteomic Investigation of Chromatin Functional Domains Reveals Novel Synergisms among Distinct Heterochromatin Components*

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The proteomic investigation of chromatin functional domains reveals novel synergisms among distinct heterochromatin components.

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