NDLI: Monosodium urate activates Src/Pyk2/PI3 kinase and cathepsin dependent unconventional protein secretion from human primary macrophages.

Please wait, while we are loading the content...

Phosphoproteomic Analysis of Protein Kinase C Signaling in Saccharomyces cerevisiae Reveals Slt2 Mitogen-activated Protein Kinase (MAPK)-dependent Phosphorylation of Eisosome Core Components

Enhanced phase II detoxification contributes to beneficial effects of dietary restriction as revealed by multi-platform metabolomics studies.

Two distinct populations of exosomes are released from LIM1863 colon carcinoma cell-derived organoids.

The Escherichia coli peripheral inner membrane proteome.

A proteomics and transcriptomics approach to identify leukemic stem cell (LSC) markers.

A high-throughput assay for phosphoprotein-specific phosphatase activity in cellular extracts.

Deletion of cysteine cathepsins B or L yields differential impacts on murine skin proteome and degradome.

A critical assessment of information-guided protein-protein docking predictions.

Subcellular distribution and dynamics of active proteasome complexes unraveled by a workflow combining in vivo complex cross-linking and quantitative proteomics.

Matrix assisted ionization vacuum (MAIV), a new ionization method for biological materials analysis using mass spectrometry.

Global subcellular characterization of protein degradation using quantitative proteomics.

Differential evolution and neofunctionalization of snake venom metalloprotease domains.

Identification of CRM1-dependent Nuclear Export Cargos Using Quantitative Mass Spectrometry

Large-scale identification of endogenous secretory peptides using electron transfer dissociation mass spectrometry.

Tools for label-free peptide quantification.

The DegraBase: a database of proteolysis in healthy and apoptotic human cells.

Metabolomic analysis of key regulatory metabolites in hepatitis C virus-infected tree shrews.

Refined preparation and use of anti-diglycine remnant (K-Îµ-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.

Linking post-translational modifications and variation of phenotypic traits.

Identification of the hypoxia-inducible factor 2 nuclear interactome in melanoma cells reveals master proteins involved in melanoma development.

A non-parametric cutout index for robust evaluation of identified proteins.

Monosodium urate activates Src/Pyk2/PI3 kinase and cathepsin dependent unconventional protein secretion from human primary macrophages.

The proteomic investigation of chromatin functional domains reveals novel synergisms among distinct heterochromatin components.

Year: 2013 Volume: 12 Issue: 2

Year: 2013 Volume: 12 Issue: 1

Year: 2012 Volume: 11

Year: 2011 Volume: 10

Monosodium urate activates Src/Pyk2/PI3 kinase and cathepsin dependent unconventional protein secretion from human primary macrophages.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Välimäki, Elina Miettinen, Juho J. Lietzén, Niina Matikainen, Sampsa Nyman, Tuula A.
Description	Country affiliation: Finland Author Affiliation: Välimäki E ( Institute of Biotechnology, University of Helsinki, University of Helsinki, Finland.)
Abstract	Monosodium urate (MSU) is an endogenous danger signal that is crystallized from uric acid released from injured cells. MSU is known to activate inflammatory response in macrophages but the molecular mechanisms involved have remained uncharacterized. Activated macrophages start to secrete proteins to activate immune response and to recruit other immune cells to the site of infection and/or tissue damage. Secretome characterization after activation of innate immune system is essential to unravel the details of early phases of defense responses. Here, we have analyzed the secretome of human primary macrophages stimulated with MSU using quantitative two-dimensional gel electrophoresis based proteomics as well as high-throughput qualitative GeLC-MS/MS approach combining protein separation by SDS-PAGE and protein identification by liquid chromatography-MS/MS. Both methods showed that MSU stimulation induced robust protein secretion from lipopolysaccharide-primed human macrophages. Bioinformatic analysis of the secretome data showed that MSU stimulation strongly activates unconventional, vesicle mediated protein secretion. The unconventionally secreted proteins included pro-inflammatory cytokines like IL-1ß and IL-18, interferon-induced proteins, and danger signal proteins. Also active forms of lysosomal proteases cathepsins were secreted on MSU stimulation, and cathepsin activity was essential for MSU-induced unconventional protein secretion. Additionally, proteins associated to phosphorylation events including Src family tyrosine kinases were increased in the secretome of MSU-stimulated cells. Our functional studies demonstrated that Src, Pyk2, and PI3 kinases act upstream of cathepsins to activate the overall protein secretion from macrophages. In conclusion, we provide the first comprehensive characterization of protein secretion pathways activated by MSU in human macrophages, and reveal a novel role for cathepsins and Src, Pyk2, PI3 kinases in the activation of unconventional protein secretion.
File Format	HTM / HTML
ISSN	15359476
e-ISSN	15359484
DOI	10.1074/mcp.M112.024661
Journal	Molecular & Cellular Proteomics
Issue Number	3
Volume Number	12
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2013-03-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Proteomics Cathepsins Metabolism Macrophages Drug Effects Phosphotransferases Proteome Secretion Uric Acid Pharmacology Amino Acid Sequence Antioxidants Cathepsin B Antagonists & Inhibitors Cells, Cultured Chemokines Chromatography, Liquid Cytokines Dipeptides Electrophoresis, Gel, Two-dimensional Enzyme Activation Focal Adhesion Kinase 2 Cytology Phosphatidylinositol 3-kinases Proteomics Tandem Mass Spectrometry Src-family Kinases Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine Analytical Chemistry Molecular Biology Biochemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Monosodium Urate Activates Src/Pyk2/PI3 Kinase and Cathepsin Dependent Unconventional Protein Secretion From Human Primary Macrophages*

Phosphatidylinositol 3-kinase-gamma activates Bruton's tyrosine kinase in concert with Src family kinases.

Dietary Salt Activates An Endothelial Pyk2/c-Src/PI3-Kinase Complex To Promote Endothelial NO Synthase Phosphorylation

TEL/platelet-derived growth factor receptor β activates phosphatidylinositol 3 (PI3) kinase and requires PI3 kinase to regulate the cell cycle

Identification of focal adhesion kinase (FAK) and phosphatidylinositol 3-kinase (PI3-kinase) as Par3 partners by proteomic analysis.

Tyrosine phosphorylation of type Iγ phosphatidylinositol phosphate kinase by Src regulates an integrin–talin switch

Phosphatidylinositol 3-kinase-γ activates Bruton’s tyrosine kinase in concert with Src family kinases

Mechanism by which cAMP activates PI3-kinase and increases bile acid secretion in WIF-B9 cells.

Ethanol Augments Monosodium Urate-Induced NLRP3 Inflammasome Activation via Regulation of AhR and TXNIP in Human Macrophages.

Monosodium urate activates Src/Pyk2/PI3 kinase and cathepsin dependent unconventional protein secretion from human primary macrophages.

Similar Documents

Monosodium Urate Activates Src/Pyk2/PI3 Kinase and Cathepsin Dependent Unconventional Protein Secretion From Human Primary Macrophages*

Phosphatidylinositol 3-kinase-gamma activates Bruton's tyrosine kinase in concert with Src family kinases.

Dietary Salt Activates An Endothelial Pyk2/c-Src/PI3-Kinase Complex To Promote Endothelial NO Synthase Phosphorylation

TEL/platelet-derived growth factor receptor β activates phosphatidylinositol 3 (PI3) kinase and requires PI3 kinase to regulate the cell cycle

Identification of focal adhesion kinase (FAK) and phosphatidylinositol 3-kinase (PI3-kinase) as Par3 partners by proteomic analysis.

Tyrosine phosphorylation of type Iγ phosphatidylinositol phosphate kinase by Src regulates an integrin–talin switch

Phosphatidylinositol 3-kinase-γ activates Bruton’s tyrosine kinase in concert with Src family kinases

Mechanism by which cAMP activates PI3-kinase and increases bile acid secretion in WIF-B9 cells.

Ethanol Augments Monosodium Urate-Induced NLRP3 Inflammasome Activation via Regulation of AhR and TXNIP in Human Macrophages.

Monosodium urate activates Src/Pyk2/PI3 kinase and cathepsin dependent unconventional protein secretion from human primary macrophages.