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Mass spectrometry-based identification and characterisation of lysine and arginine methylation in the human proteome

Content Provider	Royal Society of Chemistry (RSC)
Author	Cuomo, Alessandro Bremang, Michael Stugiewicz, Magdalena Spadotto, Valeria Agresta, Anna Maria Bonaldi, Tiziana
Copyright Year	2013
Abstract	Protein methylation is a post-translational modification (PTM) by which a variable number of methyl groups are transferred to lysine and arginine residues within proteins. Despite increased interest in this modification due to its reversible nature and its emerging role in a diverse set of biological pathways beyond chromatin, global identification of protein methylation has remained an unachieved goal. To characterise sites of lysine and arginine methylation beyond histones, we employed an approach that combines heavy methyl stable isotope labelling by amino acids in cell culture (hmSILAC) with high-resolution mass spectrometry-based proteomics. Through a broad evaluation of immuno-affinity enrichment and the application of two classical protein separation techniques prior to mass spectrometry, to nucleosolic and cytosolic fractions separately, we identified a total of 501 different methylation types, on 397 distinct lysine and arginine sites, present on 139 unique proteins. Our results considerably extend the number of known in vivo methylation sites and indicate their significant presence on several protein complexes involved at all stages of gene expression, from chromatin remodelling and transcription to splicing and translation. In addition, we describe the potential of the hmSILAC approach for accurate relative quantification of methylation levels between distinct functional states.
Starting Page	2231
Ending Page	2247
Page Count	17
File Format	HTM / HTML PDF
ISSN	1742206X
Volume Number	9
Issue Number	9
Journal	Molecular BioSystems
DOI	10.1039/c3mb00009e
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Post-translational modification Cell culture Chromatin Isotope PTM Protein Arginine Gene Lysine Classical mechanics Proteomics Methylation Mass spectrometry
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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