NDLI: Laurencia okamurai extract containing laurinterol induces apoptosis in melanoma cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kim, Moon-Moo Mendis, Eresha Kim, Se-Kwon
Description	Author Affiliation: Kim MM ( Department of Chemistry, Dong-Eui University, Busan, Republic of Korea.)
Abstract	Laurinterol is a marine sesquiterpene that has been known to have antimicrobial activity. The purpose of this study is to investigate the effect of Laurencia okamurai extract containing laurinterol (LOEL) on induction of apoptosis in melanoma cells (B16F1). Anticancer activity of LOEL against melanoma cells was shown in a dose-dependent manner by the 1-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay. It was for the first time found that LOEL exhibited an excellent effect on the induction of apoptosis as determined by DNA fragmentation, terminal deoxynucleotidyl transferase-mediated dUTP in situ nick-end labeling assay, cell cycle analysis, and measurement of activities of several caspases in melanoma cells. It was also demonstrated that transcriptional activation of p53, a tumor suppressor gene, and activation of p21 promoter by LOEL were involved in the induction of apoptosis by reporter gene assay. In particular, western blot analysis confirmed that LOEL above 5 microg/mL significantly increased the expression level of phospho-p53, the active form. These results indicate that LOEL can induce apoptosis through a p53-dependent pathway in melanoma cells.
File Format	HTM / HTML
ISSN	1096620X
Issue Number	2
Volume Number	11
e-ISSN	15577600
Journal	Journal of Medicinal Food
Language	English
Publisher	Mary Ann Liebert, Inc.
Publisher Date	2008-06-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Nutritional Sciences Apoptosis Drug Effects Laurencia Chemistry Melanoma Pathology Plant Extracts Pharmacology Sesquiterpenes Animals Antineoplastic Agents, Phytogenic Cell Line, Tumor Dna Fragmentation In Situ Nick-end Labeling Drug Therapy Melanoma, Experimental Tumor Suppressor Protein P53 Analysis Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Nutrition and Dietetics Medicine

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