| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Trujillo, Joyce Molina-Jijón, Eduardo Medina-Campos, Omar Noel Rodríguez-Muñoz, Rafael Reyes, José Luis Loredo, María L. Barrera-Oviedo, Diana Pinzón, Enrique Rodríguez-Rangel, Daniela Saraí Pedraza-Chaverri, José |
| Description |
Country affiliation: Mexico Author Affiliation: Trujillo J ( Department of Biology, Faculty of Chemistry, National Autonomous University of Mexico (UNAM), 04510 University City, D.F., Mexico. pedraza@unam.mx.); Molina-Jijón E ( Department of Physiology, Biophysics and Neurosciences, Center for Research and Advanced Studies of the National Polytechnic Institute (Cinvestav-IPN), Mexico City, 07360, Mexico.); Medina-Campos ON ( Department of Biology, Faculty of Chemistry, National Autonomous University of Mexico (UNAM), 04510 University City, D.F., Mexico. pedraza@unam.mx.); Rodríguez-Muñoz R ( Department of Physiology, Biophysics and Neurosciences, Center for Research and Advanced Studies of the National Polytechnic Institute (Cinvestav-IPN), Mexico City, 07360, Mexico.); Reyes JL ( Department of Physiology, Biophysics and Neurosciences, Center for Research and Advanced Studies of the National Polytechnic Institute (Cinvestav-IPN), Mexico City, 07360, Mexico.); Loredo ML ( School of Medicine, Panamericana University, Mexico City, 03920, Mexico.); Barrera-Oviedo D ( Department of Pharmacology, Faculty of Medicine, National Autonomous University of Mexico (UNAM), University City, 04510, Mexico.); Pinzón E ( Animal Care Unit, Faculty of Medicine, National Autonomous University of Mexico (UNAM), University City, 04510, Mexico.); Rodríguez-Rangel DS ( Department of Biology, Faculty of Chemistry, National Autonomous University of Mexico (UNAM), 04510 University City, D.F., Mexico. pedraza@unam.mx.); Pedraza-Chaverri J ( Department of Biology, Faculty of Chemistry, National Autonomous University of Mexico (UNAM), 04510 University City, D.F., Mexico. pedraza@unam.mx.) |
| Abstract |
Curcumin is a polyphenol and cisplatin is an antineoplastic agent that induces nephrotoxicity associated with oxidative stress, apoptosis, fibrosis and decrease in renal tight junction (TJ) proteins. The potential effect of curcumin against alterations in TJ structure and function has not been evaluated in cisplatin-induced nephrotoxicity. The present study explored whether curcumin is able to prevent the cisplatin-induced fibrosis and decreased expression of the TJ and adherens junction (AJ) proteins occludin, claudin-2 and E-cadherin in cisplatin-induced nephrotoxicity. Curcumin (200 mg $kg^{−1})$ was administered in three doses, and rats were sacrificed 72 h after cisplatin administration. Curcumin was able to scavenge, in a concentration-dependent way, superoxide anion, hydroxyl radical, peroxyl radical, singlet oxygen, peroxynitrite anion, hypochlorous acid and hydrogen peroxide. Cisplatin-induced renal damage was associated with alterations in plasma creatinine, expression of neutrophil gelatinase-associated lipocalin and of kidney injury molecule-1, histological damage, increase in apoptosis, fibrosis (evaluated by transforming growth factor β1, collagen I and IV and α-smooth muscle actin expressions), increase in oxidative/nitrosative stress (evaluated by Hsp70/72 expression, protein tyrosine nitration, superoxide anion production in isolated glomeruli and proximal tubules, and protein levels of NADPH oxidase subunits $p47^{phox}$ and $gp91^{phox},$ protein kinase C β2, and Nrf2) as well as by decreased expression of occludin, claudin-2, β-catenin and E-cadherin. Curcumin treatment prevented all the above-described alterations. The protective effect of curcumin against cisplatin-induced fibrosis and decreased proteins of the TJ and AJ was associated with the prevention of glomerular and proximal tubular superoxide anion production induced by NADPH oxidase activity. |
| File Format |
HTM / HTML |
| ISSN |
20426496 |
| Issue Number |
1 |
| Volume Number |
7 |
| e-ISSN |
2042650X |
| Journal |
Food & Function |
| Language |
English |
| Publisher |
Royal Society of Chemistry |
| Publisher Date |
2016-01-01 |
| Publisher Place |
Great Britain (UK) |
| Access Restriction |
Subscribed |
| Subject Keyword |
Discipline Physiology Discipline Biochemistry Discipline Chemistry Discipline Nutritional Sciences Adherens Junctions Drug Effects Antioxidants Pharmacology Cisplatin Toxicity Curcumin Oxidative Stress Tight Junctions Animals Chemistry Biological Markers Fibrosis Drug Therapy Free Radical Scavengers Kidney Diseases Chemically Induced Male Nadph Oxidase Genetics Metabolism Protein Kinase C Beta Protein Subunits Rats Rats, Wistar Reactive Oxygen Species Superoxides Journal Article Research Support, Non-u.s. Gov't |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Medicine Food Science |
