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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Rønning, Helene Thorsen Madslien, Elisabeth Henie Asp, Tone Normann Granum, Per Einar |
| Description | Country affiliation: Norway Author Affiliation: Rønning HT ( a School of Veterinary Science , Norwegian University of Life Sciences , Oslo , Norway.); Madslien EH ( b Forsvarets forskningsinstitutt FFI , Norwegian Defence Research Establishment , Kjeller , Norway.); Asp TN ( a School of Veterinary Science , Norwegian University of Life Sciences , Oslo , Norway.); Granum PE ( a School of Veterinary Science , Norwegian University of Life Sciences , Oslo , Norway.) |
| Abstract | Lichenysin produced by 53 different Bacillus licheniformis strains has been structurally examined with a qualitative liquid chromatography-tandem mass spectrometry (LC-MS/MS) method using quadrupole-time-of-flight mass spectrometry. The same lichenysin isoforms are produced from all strains, indicating that the growth conditions have a stronger influence on the lipopeptide production than the genotype. A rapid method for the quantification of lichenysin from bacterial cell cultures with LC-MS/MS after a simple methanol extraction has been refined. For the first time commercially available lichenysin has been used as calibrant, making quantification more accurate. The trueness for C15-lichenysin has been improved to 94% using matrix-matched calibration with lichenysin compared with 30% using solvent calibration with surfactin. The quantitative method was fully validated based on Commission Decision 2002/657/EC. The LOD of the method was below 1 µg g(-1) and the repeatability ranged from 10% to 16%. |
| File Format | HTM / HTML |
| ISSN | 19440049 |
| Issue Number | 12 |
| Volume Number | 32 |
| e-ISSN | 19440057 |
| Journal | Food Additives & Contaminants: Part A |
| Language | English |
| Publisher | Taylor & Francis |
| Publisher Date | 2015-01-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Toxicology Discipline Nutritional Sciences Bacillus Chemistry Lipoproteins Isolation & Purification Peptides, Cyclic Pathogenicity Physiology Calibration Chromatography, Liquid Foodborne Diseases Prevention & Control Humans Limit Of Detection Lipopeptides Analysis Liquid-liquid Extraction Methods Methanol Molecular Structure Reference Standards Reproducibility Of Results Solvents Spectrometry, Mass, Matrix-assisted Laser Desorption-ionization Tandem Mass Spectrometry Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Chemistry Health, Toxicology and Mutagenesis Public Health, Environmental and Occupational Health Toxicology Food Science |
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