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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Tao, Ze Yang, Hao Jia, Dianlong Wan, Lin Cheng, Jingqiu Lu, Xiaofeng |
| Description | Country affiliation: China Author Affiliation: Tao Z ( Key Lab of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University, Chengdu 610041, China.) |
| Abstract | Recent studies have found that, in addition to hematopoiesis, granulocyte-macrophage colony-stimulating factor (GMCSF) plays pivotal roles in multiple immune disorders. The gene encoding Macaca mulatta GMCSF (mmGMCSF) was cloned from stimulated peripheral blood mononuclear cells (PBMCs). Concanavalin A (Con A) and mismatched allogeneic antigen-stimulation significantly increased the production of mmGMCSF by monkey PBMCs. The gene encoding mature mmGMCSF was first expressed as a soluble fusion protein in Escherichia coli, and native mmGMCSF was further prepared by protease cleavage. The recombinant mmGMCSF induced antigen-presenting dendritic cells from monkey PBMCs, suggesting a central role of mmGMCSF in the immune system of the rhesus monkey. Although the predicted mature mmGMCSF protein differs from human GMCSF (hGMCSF) at six amino acid residues, mmGMCSF showed a strong ability to support human TF-1 cell survival. Additional co-immunoprecipitation experiments revealed that mmGMCSF cross reacts with the hGMCSF receptor (hGMCSFR). In addition, the hGMCSF-neutralizing agents hGMCSFR-Fc and anti-hGMCSF antibody reduced the biological effects of mmGMCSF on TF-1 cells. These results suggest that recombinant mmGMCSF might be used for the in vitro evaluation of novel hGMCSF-neutralizing agents prior to the in vivo preclinical evaluation of these agents in the rhesus monkey model. |
| File Format | HTM / HTML |
| ISSN | 0145305X |
| Issue Number | 1 |
| Volume Number | 40 |
| e-ISSN | 18790089 |
| Journal | Developmental & Comparative Immunology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-05-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Immunology Dendritic Cells Immunology Granulocyte-macrophage Colony-stimulating Factor Metabolism Leukocytes, Mononuclear Amino Acid Sequence Animals Antibodies, Blocking Pharmacology Cell Differentiation Cell Line Cell Survival Drug Effects Cloning, Molecular Concanavalin A Gene Expression Regulation Genetics Histocompatibility Antigens Humans Lymphocyte Culture Test, Mixed Macaca Mulatta Molecular Sequence Data Receptors, Granulocyte-macrophage Colony-stimulating Factor Recombinant Proteins Sequence Alignment Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Developmental Biology Immunology |
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