NDLI: Effects of extender and equilibration time on post-thaw motility and membrane integrity of cryopreserved Gyr bull semen evaluated by CASA and flow cytometry.

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Effects of extender and equilibration time on post-thaw motility and membrane integrity of cryopreserved Gyr bull semen evaluated by CASA and flow cytometry.

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Effects of extender and equilibration time on post-thaw motility and membrane integrity of cryopreserved Gyr bull semen evaluated by CASA and flow cytometry.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Leite, Ticiano Guimarães Do Vale Filho, Vicente Ribeiro de Arruda, Rubens Paes de Andrade, André Furugen Cesar Emerick, Lucas Luz Zaffalon, Fabiane Gilli Martins, Jorge André Matias de Andrade, Venício José
Description	Country affiliation: Brazil Author Affiliation: Leite TG ( Department of Clinics and Surgery, School of Veterinary Medicine, Federal University of Minas Gerais (UFMG), Belo Horizonte, MG, Brazil.)
Abstract	The objectives of the present study were to investigate the effects of three equilibration times (0, 2, and 4h) and two extenders (TRIS or Bioxcell) for cryopreservation of bull semen. Semen from 12 Gyr bulls was cryopreserved using an automated freezing machine. There were significant interactions between equilibration times and extenders for sperm motility and membrane integrity. The control treatment (0h equilibration) had the lowest values (P<0.05) for total (MOT) and progressive motilities (PROG), and percentage of sperm with intact plasma and acrosomal membranes (IPIA), with no significant differences between extenders. Extender TRIS had greater cryoprotective action than Bioxcell, with greater MOT, PROG, IPIA at 2 and 4h, as well as the lowest proportion of damaged plasma membrane (DPM, 72.2% vs. 85.8%) for all times. Equilibration for 4h yielded the most desirable (P<0.05) for MOT, PROG, and IPIA, and the least DPM percentage (86.5, 78.0, and 72.6% for 0, 2, and 4h, respectively). Overall, the combination of TRIS and 4h of equilibration was the most desirable semen cryopreservation method, with greatest MOT, PROG, and IPIA (TRIS-T4=26.8%; BIO-T4=18.3%) and the least DPM. In conclusion, based on objective analyses, equilibration during cryopreservation was essential for maintaining motility and integrity of sperm membranes; equilibration for 4h yielded the greatest sperm survival, independent of the extender used.
File Format	HTM / HTML
ISSN	03784320
Issue Number	1-4
Volume Number	120
e-ISSN	18732232
Journal	Animal Reproduction Science
Language	English
Publisher	Elsevier
Publisher Date	2010-07-01
Publisher Place	Netherlands
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Animal science Discipline Reproductive Medicine Discipline Veterinary Medicine Discipline Zoology Cell Membrane Permeability Drug Effects Cryopreservation Cryoprotective Agents Pharmacology Semen Preservation Adverse Effects Sperm Motility Temperature Animals Calibration Cattle Methods Veterinary Flow Cytometry Image Processing, Computer-assisted Male Semen Cytology Semen Analysis Instrumentation Physiology Time Factors Comparative Study Evaluation Studies Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine Animal Science and Zoology Endocrinology Food Animals

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