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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Aksoy, Melih Akman, Orhan Lehimcioglu, Necdet Cankat Erdem, Hüseyin |
| Description | Country affiliation: Turkey Author Affiliation: Aksoy M ( Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, University of Adnan Menderes, 09016 Aydin, Turkey. aksoym@tnn.net) |
| Abstract | The effects of cholesterol-loaded cyclodextrin (CLC) treatment on the osmotic tolerance and ability to undergo the acrosome reaction of rabbit spermatozoa, with an unusually high cholesterol/phospholipid ratio in plasma membranes, were examined in two successive experiments. In the first experiment, CLC-pretreated and untreated sperm cells were exposed for 15min to one of five fructose solutions, adjusted to 20, 80, 290, 500 or 1500mOsm/L. After the anisoosmotic challenge, the integrity of sperm membranes in the CLC-supplemented (at a dose level of 3mg/120x10(6)spermatozoa) and control groups was estimated by a modified hypoosmotic swelling test (HOST) associated with a supravital eosin staining test (HE-test). In the second part of the study, the influence of cholesterol supplementation on the acrosome reaction of sperm cells stimulated by either calcium ionophore A23187 (CI) or lysophosphatidylcholine (LPC) was evaluated. CLC pretreatment increased viable and live-HOST-responsive sperm rates (P<0.01) after incubation in anisoosmotic solutions varying from 80 to 1500mOsm/L. However, CLC supplementation did not influence the percentage of HOST-responsive sperm cells (P>0.05). A significant interaction was determined between CLC pretreatment and the level of osmotic pressure in maintaining the functional and physical integrities of sperm membranes undergoing osmotic challenges. Both CI and LPC successfully induced the acrosome reaction in rabbit spermatozoa (P<0.001). Compared with CI, LPC was more effective (P<0.0001). CLC pretreatment resulted in a significant reduction (P<0.01) in the percentage of acrosome reacted sperm cells irrespective of the inducing agent, either CI or LPC. In conclusion, CLC treatment enhanced the anisoosmotic tolerance of rabbit spermatozoa and reduced their ability to undergo the acrosome reaction after stimulation by CI or LPC. |
| File Format | HTM / HTML |
| ISSN | 03784320 |
| Issue Number | 1-4 |
| Volume Number | 120 |
| e-ISSN | 18732232 |
| Journal | Animal Reproduction Science |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2010-07-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Animal science Discipline Reproductive Medicine Discipline Veterinary Medicine Discipline Zoology Acrosome Reaction Drug Effects Cholesterol Pharmacology Cyclodextrins Osmosis Animals Calcimycin Cell Membrane Permeability Physiology Chemistry Cryopreservation Methods Cryoprotective Agents Down-regulation Female Ionophores Lysophosphatidylcholines Male Osmotic Pressure Rabbits Semen Preservation Spermatozoa Metabolism Evaluation Studies Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Animal Science and Zoology Endocrinology Food Animals |
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