| Author |
Ustuner, Burcu Alcay, Selim Toker, M. Berk Nur, Zekariya Gokce, Elif Sonat, Fusun Ak Gul, Zulfiye Duman, Muhammed Ceniz, Cafer Uslu, Aydin Sagirkaya, Hakan Soylu, M. Kemal |
| Description |
Author Affiliation: Ustuner B ( Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Gorukle, Bursa 16059, Turkey. Electronic address: bbaspinar@uludag.edu.tr.); Alcay S ( Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Gorukle, Bursa 16059, Turkey.); Toker MB ( Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Gorukle, Bursa 16059, Turkey.); Nur Z ( Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Gorukle, Bursa 16059, Turkey.); Gokce E ( Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Gorukle, Bursa 16059, Turkey.); Sonat FA ( Department of Physiology, Faculty of Veterinary Medicine, Uludag University, Gorukle, Bursa 16059, Turkey.); Gul Z ( Department of Pharmacology, Faculty of Medicine, Uludag University, Gorukle, Bursa 16059, Turkey.); Duman M ( Department of Aquatic Animal Disease, Faculty of Veterinary Medicine, Uludag University, Gorukle, Bursa 16059, Turkey.); Ceniz C ( Faculty of Veterinary Medicine, Uludag University, Gorukle, Bursa 16059, Turkey.); Uslu A ( Faculty of Veterinary Medicine, Uludag University, Gorukle, Bursa 16059, Turkey.); Sagirkaya H ( Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Gorukle, Bursa 16059, Turkey.); Soylu MK ( Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Gorukle, Bursa 16059, Turkey.) |
| Abstract |
The aim of the current study was to evaluate the effects of different concentrations of rainbow trout seminal plasma (RTSP) (0.1%, 1% and 10%) in extenders containing either egg yolk or lecithin for use in Awassi ram semen cryopreservation. Pooled sperm were diluted in a two-step dilution method to a final concentration of 1/5 (semen/extender) in egg yolk or lecithin extender containing no RTSP, 0.1%, 1% or 10% RTSP (v/v). Semen samples were assessed for sperm motility, plasma membrane integrity [hypoosmotic swelling test (HOST) and Hoechst 33258] and defective acrosomes [FITC-conjugated Pisum sativum agglutinin (PSA-FITC)] at the following five time points: after dilution with extender A; after equilibration; and post-thaw at 0h, 3h and 5h. Malondialdehyde (MDA) was examined only after thawing. Freezing and thawing procedures (dilution, equilibration and post-thaw incubation at 0h, 3h and 5h) negatively affected the motility (P<0.001) and acrosome integrity (P<0.001). Additionally, freezing and thawing negatively affected the plasma membrane integrity, as determined by the HOST and Hoechst 33258 (P<0.001). The extender group affected the motility (P<0.001) and the HOST results (P<0.001). Levels of MDA in the egg yolk extender with 1% RTSP group were significantly lower than in the lecithin control group (P<0.05). In conclusion, the egg yolk extender groups that were supplemented with 10% and 1% RTSP provided greater cryoprotective effects for semen survivability during 5h incubation than the other extender groups. |
