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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Sakagami, N. Nishida, K. Misumi, K. Hirayama, Y. Yamashita, S. Hoshi, H. Misawa, H. Akiyama, K. Suzuki, C. Yoshioka, K. |
| Description | Author Affiliation: Sakagami N ( Kanagawa Prefectural Livestock Industry Technology Center, Ebina, Kanagawa 243-0417, Japan. Electronic address: sakagami.semi@pref.kanagawa.jp.); Nishida K ( Kanagawa Prefectural Livestock Industry Technology Center, Ebina, Kanagawa 243-0417, Japan.); Misumi K ( Faculty of Agriculture, National Livestock Breeding Center, Shirakawa, Fukushima 961-8511, Japan.); Hirayama Y ( Faculty of Agriculture, National Livestock Breeding Center, Shirakawa, Fukushima 961-8511, Japan.); Yamashita S ( Research Institute for the Functional Peptides, Higashine, Yamagata 999-3766, Japan.); Hoshi H ( Research Institute for the Functional Peptides, Higashine, Yamagata 999-3766, Japan.); Misawa H ( Misawa Medical Industry, Kasama, Ibaraki 309-1717, Japan.); Akiyama K ( Kanagawa Prefectural Livestock Industry Technology Center, Ebina, Kanagawa 243-0417, Japan.); Suzuki C ( National Institute of Animal Health, Tsukuba, Ibaraki 305-0856, Japan.); Yoshioka K ( National Institute of Animal Health, Tsukuba, Ibaraki 305-0856, Japan.) |
| Abstract | The aim of this study was to assess the viability of vitrified-warmed in vivo-derived pig embryos after measuring the oxygen consumption rate. Six days after artificial insemination, blastocysts were collected from gilts and vitrified by the micro volume air cooling method. The oxygen consumption rate was measured in 60 vitrified-warmed embryos, which were then cultured for 48h to assess the viability. The survival (re-expansion) rate of embryos after warming was 85.0%. The average oxygen consumption rate of embryos immediately after warming was greater in embryos which could re-expand during subsequent culture (F=0.75±0.04) than that in those which failed to re-expand (F=0.33±0.05). Moreover, the oxygen consumption rate of vitrified-warmed embryos was greater in the hatched (F=0.88±0.06) than that in the not-hatched group (F=0.53±0.04). When the oxygen consumption rate of the vitrified-warmed embryos and the numbers of viable and dead cells in embryos were determined, there was a positive correlation between the oxygen consumption rate and the number of live cells (P<0.01, r=0.538). A total of 29 vitrified embryos after warming and measuring the oxygen consumption rate were surgically transferred into uterine horns of two recipients. Both of the recipients become pregnant and farrowed 12 healthy piglets. These results demonstrate that the oxygen consumption rate of vitrified-warmed pig embryos can be related to the number of live cells and that the measurement of oxygen consumption of embryos after cryopreservation may be useful for estimating embryo survivability. |
| File Format | HTM / HTML |
| ISSN | 03784320 |
| Volume Number | 164 |
| e-ISSN | 18732232 |
| Journal | Animal Reproduction Science |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-01-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Animal science Discipline Reproductive Medicine Discipline Veterinary Medicine Discipline Zoology Embryo Transfer Veterinary Oxygen Consumption Physiology Swine Embryology Tissue And Organ Harvesting Vitrification Animals Embryo Culture Techniques Female Pregnancy Pregnancy Rate Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Animal Science and Zoology Endocrinology Food Animals |
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