NDLI: Optimization of ram semen cryopreservation using a chemically defined soybean lecithin-based extender.

Please wait, while we are loading the content...

Year: 2017 Volume: 52

Year: 2016 Volume: 51

Year: 2015 Volume: 50

Year: 2014 Volume: 49

Year: 2013 Volume: 48

Year: 2013 Volume: 48 Issue: 6

Application of reproductive biotechnology for the recovery of endangered breeds: birth of the first calf of Murciana-Levantina bovine breed derived by OPU, in vitro production and embryo vitrification.

In vitro development of bison embryos using interspecies somatic cell nuclear transfer.

The effect of lactation on post-partum uterine involution in Holstein dairy cows.

Birth of kids after artificial insemination with sex-sorted, frozen-thawed goat spermatozoa.

Optimization of ram semen cryopreservation using a chemically defined soybean lecithin-based extender.

Effect of different manganese concentrations during in vitro maturation of bovine oocytes on DNA integrity of cumulus cells and subsequent embryo development.

Sperm-TALP: an alternative extender for retrieving and diluting epididymal sperm in the domestic cat.

Characterization of the exonic regions of the JY-1 gene in zebu cattle and buffaloes.

Ultrasonographic and endocrine characterization of follicular waves in llamas with a special reference to the overlapping phenomenon during successive waves.

Gonadotropin-releasing hormone administration to dairy cows without a corpus luteum 4 weeks after calving increases reproductive performance.

Detection of glycosylated proteins in rabbit oviductal isthmus and uterine endometrium during early embryo development.

Paraoxonase (PON) 1, 2 and 3 Expression in Granulosa Cells and PON1 Activity in Follicular Fluid of Dairy Cows

Evaluation of methods to determine sperm density for the European eel, Anguilla anguilla.

Effects of feeding dry glycerol to primiparous Holstein dairy cows on follicular development, reproductive performance and metabolic parameters related to fertility during the early post-partum period.

Lectin histochemistry as a tool to identify apoptotic cells in the seminiferous epithelium of Syrian hamster (Mesocricetus auratus) subjected to short photoperiod.

Effect of meloxicam on pregnancy rate of recipient heifers following transfer of in vitro produced embryos.

Effects of vasectomy on seminal plasma alkaline phosphatase in male alpacas (Vicugña pacos).

Characterization of GFR -1-positive and GFR -1-negative spermatogonia in neonatal pig testis.

Cooled storage of canine semen: in vitro effects of different concentrations of an antibiotic combination on growth of mollicutes.

Semen collection and polymerase chain reaction-based sex determination of black-headed and straw-necked ibis.

Effect of semen collection methods on the quality of pre- and post-thawed Bali cattle (Bos javanicus) spermatozoa.

Production of oocytes of Nile tilapia (Oreochromis niloticus) for in vitro fertilization via hormonal treatments.

Use of Relief Contrast(®) objective to improve sperm morphometric analysis by Isas(®) casa system in the ram.

Effect of hCG and eCG Treatments on Prostaglandins Synthesis in the Porcine Oviduct

Study of Sperm Concentration, Seminal Plasma Composition and their Physiological Correlation in the Persian Sturgeon, Acipenser persicus

Differential expression of IGF family members in heat-stressed embryos produced in vitro from OPU-derived oocytes of Nelore (Bos indicus) and Holstein (Bos taurus) cows.

Immunolocalization of melatonin and follicle-stimulating hormone receptors in caprine ovaries and their effects during in vitro development of isolated pre-antral follicles.

Methods for equine preantral follicles isolation: quantitative aspects.

The origin of amniotic polymorphonuclear leucocytes in the mare.

Year: 2013 Volume: 48 Issue: 5

Year: 2013 Volume: 48 Issue: 4

Year: 2013 Volume: 48 Issue: 3

Year: 2013 Volume: 48 Issue: 2

Year: 2013 Volume: 48 Issue: 1

Year: 2013 Volume: 48 Issue: Supplementary 1

Year: 2012 Volume: 47

Year: 2011 Volume: 46

Year: 2010 Volume: 45

Year: 2009 Volume: 44

Year: 2008 Volume: 43

Year: 2007 Volume: 42

Optimization of ram semen cryopreservation using a chemically defined soybean lecithin-based extender.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Emamverdi, M. Zhandi, M. Zare Shahneh, A. Sharafi, M. Akbari-Sharif, A.
Description	Country affiliation: Iran Author Affiliation: Emamverdi M ( Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.)
Abstract	The purpose of the present study was to investigate the effects of a chemically defined soybean lecithin-based semen extender as a substitute for egg yolk-based extenders in ram semen cryopreservation. In this study, 28 ejaculates were collected from four Zandi rams in the breeding season and then pooled together. The pooled semen was divided into six equal aliquots and diluted with six different extenders: (i) Tris-based extender (TE) containing 0.5% (w/v) soybean lecithin (SL0.5), (ii) TE containing 1% (w/v) soybean lecithin (SL1), (iii) TE containing 1.5% (w/v) soybean lecithin (SL1.5), (iv) TE containing 2% (w/v) soybean lecithin (SL2), (v) TE containing 2.5% (w/v) soybean lecithin (SL2.5) and (vi) TE containing 20% (v/v) egg yolk (EYT). After thawing, sperm motility and motion parameters, plasma membrane and acrosome integrity, apoptosis status and mitochondrial activity were evaluated. The results shown that total and progressive motility (54.43 ± 1.33% and 25.43 ± 0.96%, respectively) were significantly higher in SL1.5 when compared to other semen extenders. Sperm motion parameters (VAP, VSL, VCL, ALH and STR) were significantly higher in SL1.5 compared to other extender, with the exception of SL1 extender. Plasma membrane integrity (48.86 ± 1.38%) was significantly higher in SL1.5 when compared to other semen extenders. Also, percentage of spermatozoa with intact acrosome in SL1.5 (85.35 ± 2.19%) extender was significantly higher than that in SL0.5, SL2.5 and EYT extenders. The results showed that the proportion of live post-thawed sperm was significantly increased in SL1.5 extender compared to SL0.5, SL2 and EYT extenders. In addition, SL1, SL1.5 and SL2.5 extenders resulted in significantly lower percentage of early-apoptotic sperm than that in EYT extender. There were no significant differences in different semen extenders for percentage of post-thawed necrotic and late-apoptotic spermatozoa. Also, the results indicated that there are slight differences for percentage of live spermatozoa with active mitochondria between extenders. In conclusion, SL1.5 extender was better than other extenders in most in vitro evaluated sperm parameters.
File Format	HTM / HTML
ISSN	09366768
Issue Number	6
Volume Number	48
e-ISSN	14390531
Journal	Reproduction in Domestic Animals
Language	English
Publisher	Wiley
Publisher Date	2013-12-01
Publisher Place	Germany
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Reproductive Medicine Discipline Veterinary Medicine Discipline Zoology Cryopreservation Veterinary Cryoprotective Agents Pharmacology Plant Lectins Chemistry Semen Preservation Semen Physiology Sheep Soybean Proteins Acrosome Animals Cell Membrane Drug Effects Methods Male Sperm Motility Spermatozoa Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Animal Science and Zoology Endocrinology Biotechnology

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in