NDLI: Porosity and cell preseeding influence electrospun scaffold maturation and meniscus integration in vitro.

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Porosity and cell preseeding influence electrospun scaffold maturation and meniscus integration in vitro.

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Porosity and cell preseeding influence electrospun scaffold maturation and meniscus integration in vitro.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Ionescu, Lara C. Mauck, Robert L.
Description	Country affiliation: United States Author Affiliation: Ionescu LC ( McKay Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.)
Abstract	Electrospinning generates fibrous scaffolds ideal for engineering soft orthopedic tissues. By modifying the electrospinning process, scaffolds with different structural organization and content can be generated. For example, fibers can be aligned in a single direction, or the porosity of the scaffold can be modified through the use of multi-jet electrospinning and the removal of sacrificial fibers. In this work, we investigated the role of fiber alignment and scaffold porosity on construct maturation and integration within in vitro meniscus defects. Further, we explored the effect of preseeding expanded meniscus fibrochondrocytes (MFCs) onto the scaffold at a high density before in vitro repair. Our results demonstrate that highly porous electropun scaffolds integrate better with a native tissue and mature to a greater extent than low-porosity scaffolds, while scaffold alignment does not influence integration or maturation. The addition of expanded MFCs to scaffolds before in vitro repair improved integration with the native tissue, but did not influence maturation. In contrast, preculture of these same scaffolds for 1 month before repair decreased integration with the native tissue, but resulted in a more mature scaffold compared to implantation of cellular scaffolds or acellular scaffolds. This work will inform scaffold selection in future in vivo studies by identifying the ideal scaffold and seeding methods for meniscus tissue engineering.
File Format	HTM / HTML
ISSN	21524947
e-ISSN	21524955
DOI	10.1089/ten.TEA.2012.0052
Journal	Tissue Engineering Part A
Issue Number	3-4
Volume Number	19
Language	English
Publisher	Mary Ann Liebert, Inc.
Publisher Date	2013-02-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Biotechnology Chondrocytes Transplantation Menisci, Tibial Cytology Physiology Polyesters Chemistry Tissue Scaffolds Animals Biocompatible Materials Chemical Synthesis Cell Adhesion Cells, Cultured Electrochemistry In Vitro Techniques Surgery Porosity Rotation Research Support, N.i.h., Extramural Research Support, U.s. Gov't, Non-p.h.s.
Content Type	Text
Resource Type	Article

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