NDLI: Design and validation of a bioreactor for simulating the cardiac niche: a system incorporating cyclic stretch, electrical stimulation, and constant perfusion.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Lu, Liang Mende, Matthias Yang, Xuegeng Körber, Heinz-Felix Schnittler, Hans-Joachim Weinert, Sönke Heubach, Jürgen Werner, Carsten Ravens, Ursula
Description	Country affiliation: Germany Author Affiliation: Lu L ( Department of Pharmacology and Toxicology, Medical Faculty Carl Gustav Carus, Dresden University of Technology, Dresden, Germany.)
Abstract	To simulate the cardiac niche, a bioreactor system was designed and constructed to incorporate cyclic stretch, rhythmic electrical stimulation, and constant perfusion. The homogeneity of surface strain distribution across the cell culture substrate was confirmed with ARAMIS deformation analysis. The proliferation marker, Ki-67, detected in human umbilical vein endothelial cells and 3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyltetrazolium bromide cytotoxicity assay performed on human atrial fibroblasts confirmed biocompatibility of this novel device. Cyclic stretch treatment for 24 h resulted in the perpendicular alignment of human atrial fibroblasts. An electrical stimulation system containing carbon electrodes was characterized by electrochemical impedance spectroscopy and charge injection/recovery studies, which indicated that increased corrosive reactions were associated with a higher input voltage and prolonged pulse duration. Field stimulation delivered through this system could induce rhythmic contractions in adult rat ventricular myocytes, with contractile characteristics similar to those paced in a standard field stimulation chamber. In conclusion, this bioreactor provides a novel tool to study the interaction between physical stimulation and cardiac cell physiology.
File Format	HTM / HTML
ISSN	21524947
Issue Number	3-4
Volume Number	19
e-ISSN	21524955
Journal	Tissue Engineering Part A
Language	English
Publisher	Mary Ann Liebert, Inc.
Publisher Date	2013-02-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Biotechnology Batch Cell Culture Techniques Instrumentation Bioreactors Electric Stimulation Myocardial Contraction Physiology Myocytes, Cardiac Physical Stimulation Tissue Engineering Action Potentials Animals Cells, Cultured Elastic Modulus Equipment Design Equipment Failure Analysis Humans Male Mechanotransduction, Cellular Rats Rats, Wistar Stem Cell Niche Stress, Mechanical Evaluation Studies Journal Article Research Support, Non-u.s. Gov't Validation Studies
Content Type	Text
Resource Type	Article

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