NDLI: Doppler optical coherence tomography of retinal circulation.

Please wait, while we are loading the content...

Year: 2012 Issue: 70

Year: 2012 Issue: 69

Year: 2012 Issue: 68

Year: 2012 Issue: 67

Sample preparation of Mycobacterium tuberculosis extracts for nuclear magnetic resonance metabolomic studies.

Use of a robot for high-throughput crystallization of membrane proteins in lipidic mesophases.

Harvesting and cryo-cooling crystals of membrane proteins grown in lipidic mesophases for structure determination by macromolecular crystallography.

Intraductal injection of LPS as a mouse model of mastitis: signaling visualized via an NF-κB reporter transgenic.

Optical recording of suprathreshold neural activity with single-cell and single-spike resolution.

Models of bone metastasis.

Isolation of lymphocytes from mouse genital tract mucosa.

Isolation of basal cells and submucosal gland duct cells from mouse trachea.

Mapping the after-effects of theta burst stimulation on the human auditory cortex with functional imaging.

Time-lapse imaging of neuroblast migration in acute slices of the adult mouse forebrain.

Fluorescent in situ hybridization on mitotic chromosomes of mosquitoes.

Studying proteolysis of cyclin B at the single cell level in whole cell populations.

Exploring arterial smooth muscle Kv7 potassium channel function using patch clamp electrophysiology and pressure myography.

Enumeration of major peripheral blood leukocyte populations for multicenter clinical trials using a whole blood phenotyping assay.

Synthesis of phase-shift nanoemulsions with narrow size distributions for acoustic droplet vaporization and bubble-enhanced ultrasound-mediated ablation.

Performing vaginal lavage, crystal violet staining, and vaginal cytological evaluation for mouse estrous cycle staging identification.

High efficiency, site-specific transfection of adherent cells with siRNA using microelectrode arrays (MEA).

Doppler optical coherence tomography of retinal circulation.

Measuring the subjective value of risky and ambiguous options using experimental economics and functional MRI methods.

Multiphoton microscopy of cleared mouse brain expressing YFP.

Using unfixed, frozen tissues to study natural mucin distribution.

Preparation of acute subventricular zone slices for calcium imaging.

Flow cytometry-based purification of S. cerevisiae zygotes.

Modeling intracerebral hemorrhage in mice: injection of autologous blood or bacterial collagenase.

In vitro synthesis of native, fibrous long spacing and segmental long spacing collagen.

Driving simulation in the clinic: testing visual exploratory behavior in daily life activities in patients with visual field defects.

Probing and Mapping Electrode Surfaces in Solid Oxide Fuel Cells

Year: 2012 Issue: 66

Year: 2012 Issue: 65

Year: 2012 Issue: 64

Year: 2012 Issue: 63

Year: 2012 Issue: 62

Year: 2012 Issue: 61

Year: 2012 Issue: 60

Year: 2012 Issue: 59

Doppler optical coherence tomography of retinal circulation.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Tan, Ou Wang, Yimin Konduru, Ranjith K. Zhang, Xinbo Sadda, SriniVas R. Huang, David
Description	Country affiliation: United States Author Affiliation: Tan O ( Department of Ophthalmology, Oregon Health and Science University, OR, USA.)
Abstract	Noncontact retinal blood flow measurements are performed with a Fourier domain optical coherence tomography (OCT) system using a circumpapillary double circular scan (CDCS) that scans around the optic nerve head at 3.40 mm and 3.75 mm diameters. The double concentric circles are performed 6 times consecutively over 2 sec. The CDCS scan is saved with Doppler shift information from which flow can be calculated. The standard clinical protocol calls for 3 CDCS scans made with the OCT beam passing through the superonasal edge of the pupil and 3 CDCS scan through the inferonal pupil. This double-angle protocol ensures that acceptable Doppler angle is obtained on each retinal branch vessel in at least 1 scan. The CDCS scan data, a 3-dimensional volumetric OCT scan of the optic disc scan, and a color photograph of the optic disc are used together to obtain retinal blood flow measurement on an eye. We have developed a blood flow measurement software called 'Doppler optical coherence tomography of retinal circulation' (DOCTORC). This semi-automated software is used to measure total retinal blood flow, vessel cross section area, and average blood velocity. The flow of each vessel is calculated from the Doppler shift in the vessel cross-sectional area and the Doppler angle between the vessel and the OCT beam. Total retinal blood flow measurement is summed from the veins around the optic disc. The results obtained at our Doppler OCT reading center showed good reproducibility between graders and methods (<10%). Total retinal blood flow could be useful in the management of glaucoma, other retinal diseases, and retinal diseases. In glaucoma patients, OCT retinal blood flow measurement was highly correlated with visual field loss (R(2)>0.57 with visual field pattern deviation). Doppler OCT is a new method to perform rapid, noncontact, and repeatable measurement of total retinal blood flow using widely available Fourier-domain OCT instrumentation. This new technology may improve the practicality of making these measurements in clinical studies and routine clinical practice.
File Format	HTM / HTML
e-ISSN	1940087X
DOI	10.3791/3524
Journal	Journal of Visualized Experiments
Issue Number	67
Language	English
Publisher	MyJove Corp.
Publisher Date	2012-09-18
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Physical Sciences Discipline Life Sciences Discipline Medicine Fourier Analysis Retinal Artery Anatomy & Histology Tomography, Optical Coherence Instrumentation Blood Flow Velocity Physiology Image Interpretation, Computer-assisted Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't Video-audio Media
Content Type	Text
Resource Type	Article

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in