NDLI: Isolation of lymphocytes from mouse genital tract mucosa.

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Isolation of lymphocytes from mouse genital tract mucosa.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Jiang, Janina Kelly, Kathleen A.
Description	Country affiliation: United States Author Affiliation: Jiang J ( Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.)
Abstract	Mucosal surfaces, including in the gastrointestinal, urogenital, and respiratory tracts, provide portals of entry for pathogens, such as viruses and bacteria. Mucosae are also inductive sites in the host to generate immunity against pathogens, such as the Peyers patches in the intestinal tract and the nasal-associated lymphoreticular tissue in the respiratory tract. This unique feature brings mucosal immunity as a crucial player of the host defense system. Many studies have been focused on gastrointestinal and respiratory mucosal sites. However, there has been little investigation of reproductive mucosal sites. The genital tract mucosa is the primary infection site for sexually transmitted diseases (STD), including bacterial and viral infections. STDs are one of the most critical health challenges facing the world today. Centers for Disease Control and Prevention estimates that there are 19 million new infectious every year in the United States. STDs cost the U.S. health care system \$17 billion every year, and cost individuals even more in immediate and life-long health consequences. In order to confront this challenge, a greater understanding of reproductive mucosal immunity is needed and isolating lymphocytes is an essential component of these studies. Here, we present a method to reproducibly isolate lymphocytes from murine female genital tracts for immunological studies that can be modified for adaption to other species. The method described below is based on one mouse.
File Format	HTM / HTML
e-ISSN	1940087X
DOI	10.3791/4391
Journal	Journal of Visualized Experiments
Issue Number	67
Language	English
Publisher	MyJove Corp.
Publisher Date	2012-09-03
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Physical Sciences Discipline Life Sciences Discipline Medicine Flow Cytometry Genitalia, Female Cytology Lymphocytes Animals Mice Research Support, N.i.h., Extramural Video-audio Media
Content Type	Text
Resource Type	Article

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