NDLI: In vivo imaging systems (IVIS) detection of a neuro-invasive encephalitic virus.

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Year: 2012 Issue: 70

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In vivo imaging systems (IVIS) detection of a neuro-invasive encephalitic virus.

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In vivo imaging systems (IVIS) detection of a neuro-invasive encephalitic virus.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Poussard, Allison Patterson, Michael Taylor, Katherine Seregin, Alexey Smith, Jeanon Smith, Jennifer Salazar, Milagros Paessler, Slobodan
Description	Country affiliation: United States Author Affiliation: Poussard A ( Experimental Pathology, University of Texas Medical Branch, TX, USA.)
Abstract	Modern advancements in imaging technology encourage further development and refinement in the way viral research is accomplished. Initially proposed by Russel and Burch in Hume's 3Rs (replacement, reduction, refinement), the utilization of animal models in scientific research is under constant pressure to identify new methodologies to reduce animal usage while improving scientific accuracy and speed. A major challenge to Hume's principals however, is how to ensure the studies are statistically accurate while reducing animal disease morbidity and overall numbers. Vaccine efficacy studies currently require a large number of animals in order to be considered statistically significant and often result in high morbidity and mortality endpoints for identification of immune protection. We utilized in vivo imaging systems (IVIS) in conjunction with a firefly bioluminescent enzyme to progressively track the invasion of the central nervous system (CNS) by an encephalitic virus in a murine model. Typically, the disease progresses relatively slowly, however virus replication is rapid, especially within the CNS, and can lead to an often, lethal outcome. Following intranasal infection of the mice with TC83-Luc, an attenuated Venezuelan equine encephalitis virus strain modified to expresses a luciferase gene; we are able to visualize virus replication within the brain at least three days before the development of clinical disease symptoms. Utilizing CNS invasion as a key encephalitic disease development endpoint we are able to quickly identify therapeutic and vaccine protection against TC83-Luc infection before clinical symptoms develop. With IVIS technology we are able to demonstrate the rapid and accurate testing of drug therapeutics and vaccines while reducing animal numbers and morbidity.
File Format	HTM / HTML
e-ISSN	1940087X
DOI	10.3791/4429
Journal	Journal of Visualized Experiments
Issue Number	70
Language	English
Publisher	MyJove Corp.
Publisher Date	2012-12-02
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Physical Sciences Discipline Life Sciences Discipline Medicine Central Nervous System Virology Encephalitis Virus, Venezuelan Equine Physiology Luminescent Measurements Animals Brain Chemistry Genetics Immunology Luciferases, Firefly Biosynthesis Mice Vaccines, Attenuated Viral Vaccines Virus Replication Research Support, N.i.h., Extramural Video-audio Media
Content Type	Text
Resource Type	Article

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