NDLI: Secretory type of recombinant thioredoxin h induces ER stress in endosperm cells of transgenic rice.

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Secretory type of recombinant thioredoxin h induces ER stress in endosperm cells of transgenic rice.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Wakasa, Yuhya Yasuda, Hiroshi Takaiwa, Fumio
Description	Country affiliation: Japan Author Affiliation: Wakasa Y ( Functional Transgenic Crops Research Unit, Genetically Modified Organism Research Center, National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki 305-8602, Japan.)
Abstract	Thioredoxin h (TRX h) functions as a reducing protein and is present in all organisms. As a new approach for inducing the endoplasmic reticulum (ER) stress, TRX h (OsTRX23) was expressed as a secretory protein using the endosperm-specific glutelin GluB-1 promoter and a signal peptide. In transgenic rice seeds, the majority of the recombinant TRX h accumulated in the ER but some was also localized to the protein body IIs (PB-IIs). The rice grain quality was dependent on the TRX h accumulation level. Increased TRX h expression resulted in aberrant phenotypes, such as chalky and shriveled features, lower seed weight and lower seed protein content. Furthermore, the accumulation of some seed storage proteins (SSPs) was significantly suppressed and the morphology of the protein bodies (PB-Is and PB-IIs) changed according to the level of TRX h. SSPs, such as 13kDa prolamin and GluA, were specifically modified via the reducing action of TRX h. These changes led to the activation of the ER stress response, which was accompanied by the expression of several chaperone proteins. Specifically, the ER stress markers BiP4 and BiP5 were significantly up-regulated by an increase in the level of TRX h. These results suggest that changes in the conformation of certain SSPs via the action of recombinant TRX h lead to an induced ER stress response in transgenic rice seeds.
File Format	HTM / HTML
ISSN	01761617
Issue Number	2
Volume Number	170
e-ISSN	16181328
Journal	Journal of Plant Physiology
Language	English
Publisher	Elsevier
Publisher Date	2013-01-15
Publisher Place	Germany
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Botany Endoplasmic Reticulum Stress Physiology Endosperm Metabolism Oryza Sativa Genetics Plants, Genetically Modified Thioredoxin h Recombinant Proteins Seed Storage Proteins Seeds Up-regulation Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science Agronomy and Crop Science

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