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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Suzuki, Go Wada, Hideo Goto, Hiromi Nakano, Akiko Oba, Haruna Deno, Takuya Rahman, Sadequr Mukai, Yasuhiko |
| Description | Country affiliation: Japan Author Affiliation: Suzuki G ( Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka, 582-8582, Japan. gsuzuki@cc.osaka-kyoiku.ac.jp) |
| Abstract | Grain hardness of wheat is determined by the hardness (Ha)-locus region, which contains three friabilin-related genes: puroindoline-a (Pina), puroindoline-b (Pinb) and GSP-1. In our previous study, we produced the transgenic rice plants harboring the large genomic fragment of the Ha-locus region of Aegilops tauschii containing Pina and GSP-1 genes by Agrobacterium-mediated transformation. To examine the effects of the transgenes in the rice endosperms, we firstly confirmed the homozygosity of the T-DNAs in four independent T2 lines by using fluorescence in situ hybridization (FISH) and DNA gel blot analyses. The transgenes, Pina and GSP-1, were stably expressed in endosperms of the T3 and T4 seeds at RNA and protein levels, indicating that the promoters and other regulatory elements on the wheat Ha-locus region function in rice, and that multigene transformation using a large genomic fragment is a useful strategy. The functional contribution of the transgene-derived friabilins to the rice endosperm structure was considered as an increase of spaces between compound starch granules, resulting in a high proportion of white turbidity seeds. |
| File Format | HTM / HTML |
| ISSN | 07217714 |
| Issue Number | 12 |
| Volume Number | 30 |
| e-ISSN | 1432203X |
| Journal | Plant Cell Reports |
| Language | English |
| Publisher | Springer |
| Publisher Date | 2011-12-01 |
| Publisher Place | Germany |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Botany Oryza Sativa Metabolism Plants, Genetically Modified Triticum Genetics Agrobacterium Dna, Bacterial Dna, Plant Endosperm Ultrastructure Genes, Plant Genetic Vectors Plant Proteins Promoter Regions, Genetic Seeds Transformation, Genetic Transgenes Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Plant Science Medicine Agronomy and Crop Science |
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