NDLI: Hyaluronan-based heparin-incorporated hydrogels for generation of axially vascularized bioartificial bone tissues: in vitro and in vivo evaluation in a PLDLLA-TCP-PCL-composite system.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Rath, Subha N. Pryymachuk, Galyna Bleiziffer, Oliver A. Lam, Christopher X. F. Arkudas, Andreas Ho, Saey T. B. Beier, Justus P. Horch, Raymund E. Hutmacher, Dietmar W. Kneser, Ulrich
Description	Country affiliation: Germany Author Affiliation: Rath SN ( Department of Plastic and Hand Surgery, University of Erlangen Medical Center, Krankenhausstrasse 12, 91054, Erlangen, Germany.)
Abstract	Smart matrices are required in bone tissue-engineered grafts that provide an optimal environment for cells and retain osteo-inductive factors for sustained biological activity. We hypothesized that a slow-degrading heparin-incorporated hyaluronan (HA) hydrogel can preserve BMP-2; while an arterio-venous (A-V) loop can support axial vascularization to provide nutrition for a bio-artificial bone graft. HA was evaluated for osteoblast growth and BMP-2 release. Porous PLDLLA-TCP-PCL scaffolds were produced by rapid prototyping technology and applied in vivo along with HA-hydrogel, loaded with either primary osteoblasts or BMP-2. A microsurgically created A-V loop was placed around the scaffold, encased in an isolation chamber in Lewis rats. HA-hydrogel supported growth of osteoblasts over 8 weeks and allowed sustained release of BMP-2 over 35 days. The A-V loop provided an angiogenic stimulus with the formation of vascularized tissue in the scaffolds. Bone-specific genes were detected by real time RT-PCR after 8 weeks. However, no significant amount of bone was observed histologically. The heterotopic isolation chamber in combination with absent biomechanical stimulation might explain the insufficient bone formation despite adequate expression of bone-related genes. Optimization of the interplay of osteogenic cells and osteo-inductive factors might eventually generate sufficient amounts of axially vascularized bone grafts for reconstructive surgery.
File Format	HTM / HTML
ISSN	09574530
Issue Number	5
Volume Number	22
e-ISSN	15734838
Journal	Journal of Materials Science: Materials in Medicine
Language	English
Publisher	Springer
Publisher Date	2011-05-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Biomedical Engineering Calcium Phosphates Chemistry Heparin Hydrogels Polyesters Animals Bone Demineralization Technique Bone Morphogenetic Protein 2 Pharmacology Bone Substitutes Humans Hyaluronic Acid Male Neovascularization, Physiologic Drug Effects Osteoblasts Physiology Rats Rats, Inbred Lew Tissue Scaffolds Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Biomaterials Biophysics Bioengineering Biomedical Engineering

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