NDLI: Improved polyhydroxybutyrate (PHB) production in transgenic tobacco by enhancing translation efficiency of bacterial PHB biosynthetic genes.

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Improved polyhydroxybutyrate (PHB) production in transgenic tobacco by enhancing translation efficiency of bacterial PHB biosynthetic genes.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Matsumoto, Ken'ichiro Morimoto, Kenjiro Gohda, Aoi Shimada, Hiroaki Taguchi, Seiichi
Description	Country affiliation: Japan Author Affiliation: Matsumoto K ( Division of Biotechnology and Macromolecular Chemistry, Hokkaido University, N13-W8 Kita-ku, Sapporo 060-8628, Japan. mken@eng.hokudai.ac.jp)
Abstract	Polyhydroxybutyrate [P(3HB)] was produced in the transgenic tobacco harboring the genes encoding acetoacetyl-CoA reductase (PhaB) and polyhydroxyalkanoate synthase (PhaC) from Ralstonia eutropha (Cupriavidus necator) with optimized codon usage for expression in tobacco. P(3HB) contents in the transformants (0.2mg/g dry cell weight in average) harboring the codon-optimized phaB gene was twofold higher than the control transformants harboring the wild-type phaB gene. The immunodetection revealed an increased production of PhaB in leaves, indicating that the enhanced expression of PhaB was effective to increase P(3HB) production in tobacco. In contrast, codon-optimization of the phaC gene exhibited no apparent effect on P(3HB) production. This result suggests that the efficiency of PhaB-catalyzed reaction contributed to the flux toward P(3HB) biosynthesis in tobacco leaves.
File Format	HTM / HTML
ISSN	13891723
Issue Number	4
Volume Number	111
e-ISSN	13474421
Journal	Journal of Bioscience and Bioengineering
Language	English
Publisher	Elsevier
Publisher Date	2011-04-01
Publisher Place	Japan
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Biomedical Engineering Discipline Microbiology Acyltransferases Genetics Alcohol Oxidoreductases Genes, Bacterial Hydroxybutyrates Metabolism Tobacco Codon Cupriavidus Necator Enzymology Plant Leaves Plants, Genetically Modified Polyesters Protein Biosynthesis Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Bioengineering Applied Microbiology and Biotechnology Biotechnology

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