NDLI: Application of random mutagenesis to enhance the production of polyhydroxyalkanoates by Cupriavidus necator H16 on waste frying oil.

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Application of random mutagenesis to enhance the production of polyhydroxyalkanoates by Cupriavidus necator H16 on waste frying oil.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Obruca, Stanislav Snajdar, Ondrej Svoboda, Zdenek Marova, Ivana
Description	Author Affiliation: Obruca S ( Materials Research Centre, Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00, Brno, Czech Republic, Stana.O@seznam.cz.)
Abstract	Using random chemical mutagenesis we obtained the mutant of Cupriavidus necator H16 which was capable of improved (about 35 %) production of poly(3-hydroxybuytrate) (PHB) compared to the wild-type strain. The mutant exhibited significantly enhanced specific activities of enzymes involved in oxidative stress response such as malic enzyme, NADP-dependent isocitrate dehydrogenase, glucose-6-phosphate dehydrogenase and glutamate dehydrogenase. Probably, due to the activation of these enzymes, we also observed an increase of NADPH/NADPâº ratio. It is likely that as a side effect of the increase of NADPH/NADPâº ratio the activity of PHB biosynthetic pathway was enhanced, which supported the accumulation of PHB. Furthermore, the mutant was also able to incorporate propionate into copolymer poly(3-hydroxybuytyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] more efficiently than the wild-type strain (Y3HV/prec = 0.17 and 0.29 for the wild-type strain and the mutant, respectively)). We assume that it may be caused by lower availability of oxaloacetate for the utilization of propionyl-CoA in 2-methylcitrate cycle due to increased action of malic enzyme. Therefore, propionyl-CoA was incorporated into copolymer rather than transformed to pyruvate via 2-methylcitrate cycle. Thus, the mutant was capable of the utilization of waste frying oils and the production of P(3HB-co-3HV) with better yields and improved content of 3HV resulting in better mechanical properties of copolymer than the wild-type strain. The results of this work may be used for the development of innovative fermentation strategies for the production of PHA and also it might help to define novel targets for the genetic manipulations of PHA producing bacteria.
File Format	HTM / HTML
ISSN	09593993
Issue Number	12
Volume Number	29
e-ISSN	15730972
Journal	World Journal of Microbiology and Biotechnology
Language	English
Publisher	Springer
Publisher Date	2013-12-01
Publisher Place	Germany
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Research Support, Non-u.s. Gov't Discipline Microbiology Oxidative Stress Biosynthetic Pathways Fatty Acids, Monounsaturated Metabolism Fermentation Drug Effects Industrial Waste Journal Article Discipline Biotechnology Hydroxybutyrates Bacterial Proteins Brassica Rapa Chemistry Mutagenesis Bioreactors Polyesters Genetics Plant Oils Cupriavidus Necator Polyhydroxyalkanoates Acyl Coenzyme A
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Applied Microbiology and Biotechnology Biotechnology

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Application of random mutagenesis to enhance the production of polyhydroxyalkanoates by Cupriavidus necator H16 on waste frying oil

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Application of random mutagenesis to enhance the production of polyhydroxyalkanoates by Cupriavidus necator H16 on waste frying oil.

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Application of random mutagenesis to enhance the production of polyhydroxyalkanoates by Cupriavidus necator H16 on waste frying oil.