NDLI: A plant-produced H1N1 trimeric hemagglutinin protects mice from a lethal influenza virus challenge

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A plant-produced H1N1 trimeric hemagglutinin protects mice from a lethal influenza virus challenge

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A plant-produced H1N1 trimeric hemagglutinin protects mice from a lethal influenza virus challenge

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Shoji, Yoko Jones, R. Mark Mett, Vadim Chichester, Jessica A. Musiychuk, Konstantin Sun, Xiangjie Tumpey, Terrence M. Green, Brian J. Shamloul, Moneim Norikane, Joey Bi, Hong Hartman, Caitlin E. Bottone, Cory Stewart, Michelle Streatfield, Stephen J. Yusibov, Vidadi
Description	Author Affiliation: Shoji Y ( Fraunhofer USA Center for Molecular Biotechnology)
Abstract	The increased worldwide awareness of seasonal and pandemic influenza, including pandemic H1N1 virus, has stimulated interest in the development of economic platforms for rapid, large-scale production of safe and effective subunit vaccines. In recent years, plants have demonstrated their utility as such a platform and have been used to produce vaccine antigens against various infectious diseases. Previously, we have produced in our transient plant expression system a recombinant monomeric hemagglutinin (HA) protein (HAC1) derived from A/California/04/09 (H1N1) strain of influenza virus and demonstrated its immunogenicity and safety in animal models and human volunteers. In the current study, to mimic the authentic HA structure presented on the virus surface and to improve stability and immunogenicity of the HA antigen, we generated trimeric HA by introducing a trimerization motif from a heterologous protein into the HA sequence. Here, we describe the engineering, production in Nicotiana benthamiana plants, and characterization of the highly purified recombinant trimeric HA protein (tHA-BC) from A/California/04/09 (H1N1) strain of influenza virus. The results demonstrate the induction of serum hemagglutination inhibition antibodies by tHA-BC and its protective efficacy in mice against a lethal viral challenge. In addition, the immunogenic and protective doses of tHA-BC were much lower compared with monomeric HAC1. Further investigation into the optimum vaccine dose and/or regimen as well as the stability of trimerized HA is necessary to determine whether trimeric HA is a more potent vaccine antigen than monomeric HA.
File Format	HTM / HTML
ISSN	21645515
e-ISSN	2164554X
Journal	Human Vaccines & Immunotherapeutics
Issue Number	3
Volume Number	9
Language	English
Publisher	Taylor & Francis
Publisher Date	2013-03-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Immunology Discipline Therapeutics Hemagglutinin Glycoproteins, Influenza Virus Administration & Dosage Immunology Influenza A Virus, H1n1 Subtype Influenza Vaccines Orthomyxoviridae Infections Prevention & Control Animals Antibodies, Bacterial Blood Disease Models, Animal Hemagglutination Inhibition Tests Genetics Isolation & Purification Mice, Inbred Balb C Plants, Genetically Modified Protein Engineering Protein Multimerization Survival Analysis Tobacco Vaccines, Subunit Vaccines, Synthetic Research Support, U.s. Gov't, Non-p.h.s.
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology Pharmacology

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A plant-produced H1N1 trimeric hemagglutinin protects mice from a lethal influenza virus challenge