NDLI: Anti-proliferative and apoptotic effects of the novel taspine derivative tas41 in the Caco-2 cell line.

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Anti-proliferative and apoptotic effects of the novel taspine derivative tas41 in the Caco-2 cell line.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Zhang, Yanmin Zhang, Jie Dai, Bingling Wang, Nan He, Langchong
Description	Author Affiliation: Zhang Y ( School of Medicine, Xi'an Jiaotong University, No. 76, Yanta Weststreet, #120, Xi'an, Shaanxi Province 710061, PR China.)
Abstract	Taspine was screened and isolated for the first time from Radix et Rhizoma Leonticis. Tas41 is a novel taspine derivative. We investigated the effects of tas41 on proliferation of the Caco-2 cell line using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), a fluorescence-activated cell sorter (FACS), enzyme-linked immunosorbent assay (ELISA), reverse transcription-polymerase chain reaction (RT-PCR) and western blotting (WB). Changes in the cell cycle, apoptosis, activation of caspase-3, caspase-8 and caspase-9, and expressions of vascular endothelial growth factor (VEGF) and epidermal growth factor (EGF) were investigated after Caco-2 cells were treated with tas41. At the same time, expressions of apoptosis protein bcl-2 and bax were determined. Tas41 was found to induce apoptosis in a concentration-dependent manner as confirmed by DNA fragmentation analysis, TUNEL assay and flow cytometry. Protein and mRNA expressions of EGF, VEGF, CDK2, bcl-2 and bax were evaluated by ELISA, WB and RT-PCR. Tas41 had a better anti-proliferative effect than taspine on Caco-2 cells. A DNA ladder and apoptosis was observed, and the increased apoptotic activity by tas41 was accompanied by a decrease in the expression of VEGF protein and mRNA. The activities of caspase-3, caspase-8 and caspase-9 were significantly increased in cells treated with tas41 compared with those in the control group. In addition, protein and mRNA expressions of bcl-2 were decreased, and protein and mRNA expressions of bax were increased. These findings demonstrate that tas41 can inhibit the proliferation of, and induce apoptosis in, Caco-2 cells by activating caspase-3, caspase-8 and caspase-9, downregulating the expressions of VEGF, upregulating the ratio of bax/bcl-2.
File Format	HTM / HTML
ISSN	13826689
Issue Number	3
Volume Number	31
e-ISSN	18727077
Journal	Environmental Toxicology and Pharmacology
Language	English
Publisher	Elsevier
Publisher Date	2011-05-01
Publisher Place	Netherlands
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Environmental Health Discipline Pharmacology Alkaloids Pharmacology Antineoplastic Agents, Phytogenic Apoptosis Drug Effects Morpholines Chemistry Blotting, Western Caco-2 Cells Caspase 3 Metabolism Caspase 8 Caspase 9 Cell Cycle Cell Proliferation Cell Survival Cyclin D1 Biosynthesis Dna Fragmentation Down-regulation Flow Cytometry Humans In Situ Nick-end Labeling Rna Isolation & Purification Reverse Transcriptase Polymerase Chain Reaction Up-regulation Vascular Endothelial Growth Factor A Genetics Bcl-2-associated X Protein Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Health, Toxicology and Mutagenesis Medicine Toxicology Pharmacology

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