| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Zheng, WangLong Pan, ShunYe Wang, Guangguang Wang, Ya Jun Liu, Qing Gu, JianHong Yuan, Yan Liu, Xue Zhong Liu, Zong Ping Bian, Jian Chun |
| Description |
Country affiliation: China Author Affiliation: Zheng W ( College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China); Pan S ( College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China); Wang G ( College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China); Wang YJ ( College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China); Liu Q ( Jiaozuo Entry-Exit Inspection and Quarantine Bureau of P.R. China, Jiaozuo 454001, Henan, China.); Gu J ( College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China); Yuan Y ( College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China); Liu XZ ( College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China); Liu ZP ( College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China); Bian JC ( College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China) |
| Abstract |
The aim of this study was to investigate the effects of ZEA on the cytoskeletal structure, and factors specifically expressed by Sertoli cells. Primary Sertoli cells from rats aged 18-21 days were exposed to increasing ZEA concentrations (0, 5, 10, 20 µg mL(-1)) for 24 h. The results of immunofluorescence showed disruption of -tubulin filaments and F-actin bundles, and damage to the nucleus of Sertoli cells on exposure to ZEA. In the control group, the protein level expression of androgen-binding protein (ABP), transferrin, vimentin, N-cadherin, and follicle-stimulating hormone receptor (FSHR) were decreased significantly (p<0.05, p<0.01). The mRNA levels of ABP, transferrin, vimentin, N-cadherin, and FSHR varied significantly in the experimental group (p<0.05). The results of enzyme-linked immunosorbent assay indicated a significant decrease in the levels of inhibin-ß and transferrin in the cultural supernatants (p<0.05). Additionally, the ultrastructural analysis indicated the absence of mitochondria and Golgi apparatus, and presence of vacuoles in the cytoplasm. These findings showed that ZEA treatment can damage the cytoskeletal structure and affect specific secretory functions of Sertoli cells, which may be an underlying cause of ZEA-induced reproductive toxicity. |
| File Format |
HTM / HTML |
| ISSN |
13826689 |
| Journal |
Environmental Toxicology and Pharmacology |
| Volume Number |
42 |
| e-ISSN |
18727077 |
| Language |
English |
| Publisher |
Elsevier |
| Publisher Date |
2016-03-01 |
| Publisher Place |
Netherlands |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Discipline Environmental Health Discipline Pharmacology |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Health, Toxicology and Mutagenesis Medicine Toxicology Pharmacology |
