| Author |
Cariddi, L. N. Escobar, F. M. Sabini, M. C. Campra, N. A. Bagnis, G. Decote-Ricardo, D. Freire-de-Lima, C. G. Mañas, F. Sabini, L. I. Dalcero, A. M. |
| Description |
Author Affiliation: Cariddi LN ( Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Ruta 36 Km 601, Río Cuarto, CP 5800 Córdoba, Argentina); Escobar FM ( Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Ruta 36 Km 601, Río Cuarto, CP 5800 Córdoba, Argentina); Sabini MC ( Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Ruta 36 Km 601, Río Cuarto, CP 5800 Córdoba, Argentina); Campra NA ( Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Ruta 36 Km 601, Río Cuarto, CP 5800 Córdoba, Argentina.); Bagnis G ( Departamento de Patología Animal, Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Ruta 36 Km 601, Río Cuarto, CP 5800 Córdoba, Argentina.); Decote-Ricardo D ( Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.); Freire-de-Lima CG ( Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.); Mañas F ( Cátedra de Farmacología, Universidad Nacional de Río Cuarto, Ruta 36 Km 601, Río Cuarto, CP 5800 Córdoba, Argentina.); Sabini LI ( Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Ruta 36 Km 601, Río Cuarto, CP 5800 Córdoba, Argentina.); Dalcero AM ( Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Ruta 36 Km 601, Río Cuarto, CP 5800 Córdoba, Argentina) |
| Abstract |
The present study aimed to characterize the chlorogenic acid (ChlA) capacity to reverse the toxic effects induced by ochratoxin A (OTA) in a subacute toxicity test in rats. Male Wistar rats were fed orally by gavage for 28 days with OTA (0.4mg/kg bw/day), ChlA (5mg/kg bw/day) or the combination OTA (0.4mg/kg bw/day)+ChlA (5mg/kg bw/day). No deaths, no decrease in feed intake or body weight in any experimental group were recorded. The negative control group and the animals treated with ChlA alone showed no changes in any parameters evaluated. In OTA-treated group significant changes such as decrease in urine volume, proteinuria, occult blood, increase in serum creatinine values; decrease in absolute and relative kidney weight and characteristics histopathological lesions that indicated kidney damage were observed. However, limited effect on oxidative stress parameters were detected in kidneys of OTA-treated group. Animals treated with the combination OTA+ChlA were showed as negative control group in the evaluation of several parameters of toxicity. In conclusion, ChlA, at given concentration, improved biochemical parameters altered in urine and serum and pathological damages in kidneys induced by OTA exposure, showing a good protective activity, but not by an apparent antioxidant mechanism. |
