| Author |
Fernando, Pattage Madushan Dilhara Jayatissa Piao, Mei Jing Hewage, Susara Ruwan Kumara Madduma Kang, Hee Kyoung Yoo, Eun Sook Koh, Young Sang Ko, Mi Hee Ko, Chang Sik Byeon, Sang Hee Mun, Seung Ri Lee, Nam Ho Hyun, Jin Won |
| Description |
Author Affiliation: Fernando PM ( Department of Biochemistry, School of Medicine, Jeju National University, Jeju 63243, Republic of Korea.); Piao MJ ( Department of Biochemistry, School of Medicine, Jeju National University, Jeju 63243, Republic of Korea.); Hewage SR ( Department of Biochemistry, School of Medicine, Jeju National University, Jeju 63243, Republic of Korea.); Kang HK ( Department of Biochemistry, School of Medicine, Jeju National University, Jeju 63243, Republic of Korea.); Yoo ES ( Department of Biochemistry, School of Medicine, Jeju National University, Jeju 63243, Republic of Korea.); Koh YS ( Department of Biochemistry, School of Medicine, Jeju National University, Jeju 63243, Republic of Korea.); Ko MH ( Jeju Biodiversity Research Institute, Jeju Technopark, Jeju 63243, Republic of Korea.); Ko CS ( Jeju Biodiversity Research Institute, Jeju Technopark, Jeju 63243, Republic of Korea.); Byeon SH ( Department of Chemistry and Cosmetics, College of Natural Sciences, Jeju National University, Jeju 63243, Republic of Korea.); Mun SR ( Department of Chemistry and Cosmetics, College of Natural Sciences, Jeju National University, Jeju 63243, Republic of Korea.); Lee NH ( Department of Chemistry and Cosmetics, College of Natural Sciences, Jeju National University, Jeju 63243, Republic of Korea.); Hyun JW ( Department of Biochemistry, School of Medicine, Jeju National University, Jeju 63243, Republic of Korea. Electronic address: jinwonh@jejunu.ac.kr.) |
| Abstract |
The aim of this study was to evaluate the photo-preventive effects of sargachromenol (SC) against ultraviolet B (UVB)-induced oxidative stress in human keratinocytes via assessing the antioxidant properties and underlying molecular mechanisms. SC exhibited a significant scavenging effect on UVB-induced intracellular reactive oxygen species (ROS). SC attenuated UVB-induced oxidative macromolecular damage, including the protein carbonyl content, DNA strand break, and 8-isoprostane level. Furthermore, SC decreased UVB-induced Bax, cleaved caspase-9, and cleaved caspase-3 protein levels, but increased that of Bcl-2, which are well-known key mediators of apoptosis. Moreover, SC increased superoxide dismutase, catalase, and heme oxygenase-1 protein expression. Pre-treatment with SC upregulated the main transcription factor of antioxidant enzymes, erythroid 2-related factor 2 level, which was reduced by UVB irradiation. Extracellular signal-regulated kinase (ERK) and Jun N-terminal kinases (JNK) are involved in the regulation of many cellular events, including apoptosis. SC treatment reversed ERK and JNK activation induced by UVB. Collectively, these data indicate that SC can provide remarkable cytoprotection against the adverse effects of UVB radiation by modulating cellular antioxidant systems, and suggest the potential of developing a medical agent for ROS-induced skin diseases. |
