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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | So, Austin Pel, Joel Rajan, Sweta Marziali, Andre |
| Description | Country affiliation: Canada Author Affiliation: So A ( Boreal Genomics Inc., Vancouver, British Columbia V6T 1Z3, Canada.) |
| Abstract | Methods for the extraction of nucleic acids are straightforward in instances where there is ample nucleic acid mass in the sample and contamination is minimal. However, applications in areas such as metagenomics, life science research, clinical research, and forensics, that are limited by smaller amounts of starting materials or more dilute samples, require sample preparation methods that are more efficient at extracting nucleic acids. Synchronous coefficient of drag alteration (SCODA) is a novel electrophoretic nucleic acid purification technology that has been tested successfully with both highly contaminated and dilute samples and is a promising candidate for new sample preparation challenges. In this article, as an example of SCODA's performance with limited sample material, we outline a genomic DNA (gDNA) extraction protocol from low abundance cultures of Escherichia coli DH10B. This method is equally well suited to high biomass samples. |
| File Format | HTM / HTML |
| ISSN | 19403402 |
| Issue Number | 10 |
| Journal | Cold Spring Harbor Protocols |
| Volume Number | 2010 |
| e-ISSN | 15596095 |
| Language | English |
| Publisher | Cold Spring Harbor Laboratory Press |
| Publisher Date | 2010-10-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Clinical Laboratory Techniques Dna Isolation & Purification Escherichia Coli Genetics Genomics Methods Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry, Genetics and Molecular Biology |
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