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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Simpson, Richard J. |
| Abstract | Bacteria are particularly convenient for producing recombinant proteins for purification purposes. Suitable extraction methods for bacterial cells include sonication, glass bead milling, grinding with alumina or sand, high-pressure shearing using the French pressure cell (French Press), and lysozyme treatment. These procedures are applicable for preparing extracts from a variety of gram-negative bacteria such as Escherichia coli and Klebsiella pneumoniae, and gram-positive bacteria such as Bacillus subtilis. Disruption of bacterial cells by enzymatic means is commonly used because a relatively uniform treatment is obtained when cells are in suspension. This protocol describes the enzymatic disruption of E. coli. |
| File Format | HTM / HTML |
| ISSN | 19403402 |
| Issue Number | 9 |
| Volume Number | 2010 |
| e-ISSN | 15596095 |
| Journal | Cold Spring Harbor Protocols |
| Language | English |
| Publisher | Cold Spring Harbor Laboratory Press |
| Publisher Date | 2010-09-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Clinical Laboratory Techniques Escherichia Coli Metabolism Gene Expression Recombinant Proteins Isolation & Purification Bacteriolysis Genetics Muramidase Protein Engineering Methods Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry, Genetics and Molecular Biology |
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