Please wait, while we are loading the content...
Please wait, while we are loading the content...
| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Bashaw, Greg J. |
| Abstract | How axons in the developing nervous system navigate to their correct targets is a fundamental question in neuroscience. Studies of axon guidance in the embryonic central nervous system (CNS) of Drosophila melanogaster have proved instrumental to the identification of molecules and mechanisms that regulate wiring. The relative simplicity of the Drosophila embryonic CNS, the advantages of genetic approaches, and the ability to analyze the pathfinding decisions of well-defined axon groups or even individual axons have all contributed to our understanding of the fundamental mechanisms of axon guidance and target selection. In this protocol, we describe methods for using fluorescent antibody staining as well as horseradish peroxidase (HRP) immunohistochemistry to reveal axon-projection patterns and identify specific guidance molecules. In the presence of 3,3'-diaminobenzidine tetrahydrochloride (DAB), HRP forms a brown, stable precipitate that allows visualization of axon populations under white light. This can be quite advantageous when teaching basic embryonic anatomy and dissection techniques, because unlike fluorescent antibody staining, HRP can be visualized in a lighted room. HRP detection does not allow precise colocalization of signals, nor can multiple colors be visualized simultaneously. These objectives are easily achieved with fluorescent antibody staining. |
| File Format | HTM / HTML |
| ISSN | 19403402 |
| e-ISSN | 15596095 |
| DOI | 10.1101/pdb.prot5503 |
| Journal | Cold Spring Harbor Protocols |
| Issue Number | 10 |
| Volume Number | 2010 |
| Language | English |
| Publisher | Cold Spring Harbor Laboratory Press |
| Publisher Date | 2010-10-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Discipline Clinical Laboratory Techniques Drosophila Cytology Fluorescent Antibody Technique Immunohistochemistry Animals Axons Central Nervous System Embryology Embryo Culture Techniques |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry, Genetics and Molecular Biology |
National Digital Library of India (NDLI) is a virtual repository of learning resources which is not just a repository with search/browse facilities but provides a host of services for the learner community. It is sponsored and mentored by Ministry of Education, Government of India, through its National Mission on Education through Information and Communication Technology (NMEICT). Filtered and federated searching is employed to facilitate focused searching so that learners can find the right resource with least effort and in minimum time. NDLI provides user group-specific services such as Examination Preparatory for School and College students and job aspirants. Services for Researchers and general learners are also provided. NDLI is designed to hold content of any language and provides interface support for 10 most widely used Indian languages. It is built to provide support for all academic levels including researchers and life-long learners, all disciplines, all popular forms of access devices and differently-abled learners. It is designed to enable people to learn and prepare from best practices from all over the world and to facilitate researchers to perform inter-linked exploration from multiple sources. It is developed, operated and maintained from Indian Institute of Technology Kharagpur.
Learn more about this project from here.
NDLI is a conglomeration of freely available or institutionally contributed or donated or publisher managed contents. Almost all these contents are hosted and accessed from respective sources. The responsibility for authenticity, relevance, completeness, accuracy, reliability and suitability of these contents rests with the respective organization and NDLI has no responsibility or liability for these. Every effort is made to keep the NDLI portal up and running smoothly unless there are some unavoidable technical issues.
Ministry of Education, through its National Mission on Education through Information and Communication Technology (NMEICT), has sponsored and funded the National Digital Library of India (NDLI) project.
| Sl. | Authority | Responsibilities | Communication Details |
|---|---|---|---|
| 1 | Ministry of Education (GoI), Department of Higher Education |
Sanctioning Authority | https://www.education.gov.in/ict-initiatives |
| 2 | Indian Institute of Technology Kharagpur | Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project | https://www.iitkgp.ac.in |
| 3 | National Digital Library of India Office, Indian Institute of Technology Kharagpur | The administrative and infrastructural headquarters of the project | Dr. B. Sutradhar bsutra@ndl.gov.in |
| 4 | Project PI / Joint PI | Principal Investigator and Joint Principal Investigators of the project |
Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon |
| 5 | Website/Portal (Helpdesk) | Queries regarding NDLI and its services | support@ndl.gov.in |
| 6 | Contents and Copyright Issues | Queries related to content curation and copyright issues | content@ndl.gov.in |
| 7 | National Digital Library of India Club (NDLI Club) | Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach | clubsupport@ndl.gov.in |
| 8 | Digital Preservation Centre (DPC) | Assistance with digitizing and archiving copyright-free printed books | dpc@ndl.gov.in |
| 9 | IDR Setup or Support | Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops | idr@ndl.gov.in |
|
Loading...
|