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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Bootman, Martin D. Rietdorf, Katja Collins, Tony Walker, Simon Sanderson, Michael |
| Description | Country affiliation: United kingdom Author Affiliation: Bootman MD ( Babraham Institute, Babraham, Cambridge, CB22 3AT, United Kingdom. martin.bootman@babraham.ac.uk) |
| Abstract | Imaging $Ca^{2+}-sensitive$ fluorescent indicators provides a common approach for studying $Ca^{2+}$ signals in many contexts. Fluorescent indicators are particularly useful for measuring acute $Ca^{2+}$ changes in a relatively noninvasive manner. The availability of indicators that can be targeted to specific cellular domains, coupled with variations in affinity, brightness or spectral characteristics, provides tools for exploring spatially and temporally diverse $Ca^{2+}$ signals, and moreover, multiplexing the readout of $Ca^{2+}$ with other cellular functions. This article aims to give the novice experimentalist some insight into the considerations and potential pitfalls that impinge on the use of fluorescent $Ca^{2+}$ indicators. |
| File Format | HTM / HTML |
| ISSN | 19403402 |
| Issue Number | 2 |
| Journal | Cold Spring Harbor Protocols |
| Volume Number | 2013 |
| e-ISSN | 15596095 |
| Language | English |
| Publisher | Cold Spring Harbor Laboratory Press |
| Publisher Date | 2013-02-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Clinical Laboratory Techniques Calcium Chemistry Fluorescent Dyes Molecular Imaging Calcium Signaling Journal Article Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry, Genetics and Molecular Biology |
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