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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Andreucci, Michele Faga, Teresa Pisani, Antonio Sabbatini, Massimo Russo, Domenico Mattivi, Fulvio De Sarro, Giovambattista Navarra, Michele Michael, Ashour |
| Description | Author Affiliation: Andreucci M ( Department of 'Health Sciences', Nephrology Unit, Magna Graecia University, Catanzaro I-88100, Italy. Electronic address: andreucci@unicz.it.); Faga T ( Department of 'Health Sciences', Nephrology Unit, Magna Graecia University, Catanzaro I-88100, Italy.); Pisani A ( Department of 'Public Health', Nephrology Unit, Federico II University, Naples I-80131, Italy.); Sabbatini M ( Department of 'Public Health', Nephrology Unit, Federico II University, Naples I-80131, Italy.); Russo D ( Department of 'Public Health', Nephrology Unit, Federico II University, Naples I-80131, Italy.); Mattivi F ( Department of Food Quality and Nutrition, Fondazione Edmund Mach, Research and Innovation Centre, San Michele all'Adige, I-38010, Italy.); De Sarro G ( Department of 'Health Sciences', Pharmacology Unit, Magna Graecia University, Catanzaro I-88100, Italy.); Navarra M ( Department of 'Drug Sciences and Products for Health', University of Messina, I-98168 Messina, Italy.); Michael A ( Department of 'Health Sciences', Nephrology Unit, Magna Graecia University, Catanzaro I-88100, Italy.) |
| Abstract | Radiocontrast media (RCM)-induced nephrotoxicity (CIN) is a major clinical problem accounting for 12% of all hospital-acquired cases of acute kidney injury. The pathophysiology of CIN is not well understood, but direct toxic effects on renal cells have been postulated as contributing to CIN. We have investigated the effect of a white grape (Vitis vinifera) juice extract (WGJe) on human renal proximal tubular (HK-2) cells treated with the radiocontrast medium (RCM) sodium diatrizoate. WGJe caused an increase in phosphorylation of the prosurvival kinases Akt and ERK1/2 in HK-2 cells. Treatment of HK-2 cells with 75 mgI/ml sodium diatrizoate for 2.5h and then further incubation (for 27.5h) after removal of the RCM caused a drastic decrease in cell viability. However, pre-treatment with WGJe, prior to incubation with diatrizoate, dramatically improved cell viability. Analysis of key signaling molecules by Western blotting showed that diatrizoate caused a drastic decrease in phosphorylation of Akt (Ser473), FOXO1 (Thr24) and FOXO3a (Thr32) during the initial 2.5h incubation period, and WGJe pre-treatment caused a reversal of these effects. Further analysis by Western blotting of samples from HK-2 cells cultured for longer periods of time (for up to 27.5h after an initial 2.5h exposure to diatrizoate with or without WGJe pre-treatment) showed that WGJe pre-treatment caused a negative effect on phosphorylation of p38, NF-κB (Ser276) and pERK1/2 whilst having a positive effect on the phosphorylation of Akt, FOXO1/FOXO3a and maintained levels of Pim-1 kinase. WGJe may alleviate RCM toxicity through modulation of signaling molecules that are known to be involved in cell death and cell survival and its possible beneficial effects should be further investigated. |
| File Format | HTM / HTML |
| ISSN | 00092797 |
| Volume Number | 229 |
| e-ISSN | 18727786 |
| Journal | Chemico-Biological Interactions |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-03-05 |
| Publisher Place | Ireland |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biochemistry Discipline Pharmacology Beverages Contrast Media Toxicity Diatrizoate Kidney Tubules, Proximal Drug Effects Plant Extracts Pharmacology Protective Agents Vitis Chemistry Acute Kidney Injury Chemically Induced Analysis Cell Line Cell Survival Forkhead Transcription Factors Metabolism Humans Cytology Pathology Phosphorylation Isolation & Purification Proto-oncogene Proteins C-akt Signal Transduction Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Toxicology |
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