NDLI: Effects of culture media on metabolic profiling of the human gastric cancer cell line SGC7901.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Huang, Zicheng Shao, Wei Gu, Jinping Hu, Xiaomin Shi, Yuanzhi Xu, Wenqi Huang, Caihua Lin, Donghai
Description	Author Affiliation: Huang Z ( High-field NMR Research Center, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, P. R. China. dhlin@xmu.edu.cn.)
Abstract	Cell culture metabolomics has demonstrated significant advantages in cancer research. However, its applications have been impeded by some influencing factors such as culture media, which could significantly affect cellular metabolic profiles and lead to inaccuracy and unreliability of comparative metabolomic analysis of cells. To evaluate the effects of different culture media on cellular metabolic profiling, we performed NMR-based metabolomic analysis of the human gastric cancer cell line SGC7901 cultured in both RPMI1640 and DMEM. We found that SGC7901 cultured in the two media exhibited distinct metabolic profiles with obviously different levels of discrepant metabolites, even though they showed almost the same cellular morphology and proliferation rates. When SGC7901 originally cultured in RPMI1640 was gradually acclimated in DMEM, both the metabolic profiles and most of the discrepant metabolite levels gradually converged toward those of the cells originally cultured in DMEM without significantly altered cell proliferation rates. However, several metabolite levels did not show the converging trends. Our results indicate that the effects of culture media on metabolic profiling must be carefully taken into account for comparative metabolomic analysis of cell lines. This work may be of benefit to the development of cell culture metabolomics.
File Format	HTM / HTML
ISSN	1742206X
Issue Number	7
Volume Number	11
e-ISSN	17422051
Journal	Molecular BioSystems
Language	English
Publisher	Royal Society of Chemistry
Publisher Date	2015-07-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Discipline Molecular Biology Discipline Biochemistry Metabolome Stomach Neoplasms Metabolism Cell Line, Tumor Cell Proliferation Cell Shape Culture Media Humans Comparative Study Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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