NDLI: Detection of rhabdovirus viral RNA in oropharyngeal swabs and ectoparasites of Spanish bats.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Aznar-Lopez, Carolina Vazquez-Moron, Sonia Marston, Denise A. Juste, Javier Ibáñez, Carlos Berciano, Jose Miguel Salsamendi, Egoitz Aihartza, Joxerra Banyard, Ashley C. McElhinney, Lorraine Fooks, Anthony R. Echevarria, Juan
Description	Country affiliation: Spain Author Affiliation: Aznar-Lopez C ( Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, Spain. carolinaaznarlopez@hotmail.com)
Abstract	Rhabdoviruses infect a variety of hosts, including mammals, birds, reptiles, fish, insects and plants. As bats are the natural host for most members of the genus Lyssavirus, the specificity of the amplification methods used for active surveillance is usually restricted to lyssaviruses. However, the presence of other rhabdoviruses in bats has also been reported. In order to broaden the scope of such methods, a new RT-PCR, able to detect a diverse range of rhabdoviruses, was designed. The method detected 81 of 86 different rhabdoviruses. In total, 1488 oropharyngeal bat swabs and 38 nycteribiid samples were analysed, and 17 unique rhabdovirus-related sequences were detected. Phylogenetic analysis suggested that those sequences detected in bats did not constitute a monophyletic group, even when originating from the same bat species. However, all of the sequences detected in nycteribiids and one sequence obtained from a bat did constitute a monophyletic group with Drosophila melanogaster sigma rhabdovirus.
File Format	HTM / HTML
ISSN	00221317
Issue Number	Pt 1
Volume Number	94
e-ISSN	14652099
Journal	Journal of General Virology
Language	English
Publisher	Microbiology Society
Publisher Date	2013-01-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Research Support, Non-u.s. Gov't Discipline Virology Isolation & Purification Oropharynx Rna, Viral Phylogeny Reverse Transcriptase Polymerase Chain Reaction Journal Article Virology Rhabdoviridae Infections Drosophila Melanogaster Animals Insects Rhabdoviridae Analysis Genetics Chiroptera Methods
Content Type	Text
Resource Type	Article
Subject	Virology

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