NDLI: Bombyx mori nucleopolyhedrovirus nucleic acid binding proteins BRO-B and BRO-E associate with host T-cell intracellular antigen 1 homologue BmTRN-1 to influence protein synthesis during infection.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kotani, Eiji Muto, Sayaka Ijiri, Hiroshi Mori, Hajime
Description	Country affiliation: Japan Author Affiliation: Kotani E ( 1Department of Applied Biology, Kyoto Institute of Technology, Sakyo-ku, Kyoto 606-8585, Japan 2Insect Biomedical Center, Kyoto Institute of Technology, Sakyo-ku, Kyoto 606-8585, Japan.); Muto S ( 1Department of Applied Biology, Kyoto Institute of Technology, Sakyo-ku, Kyoto 606-8585, Japan.); Ijiri H ( 2Insect Biomedical Center, Kyoto Institute of Technology, Sakyo-ku, Kyoto 606-8585, Japan.); Mori H ( 1Department of Applied Biology, Kyoto Institute of Technology, Sakyo-ku, Kyoto 606-8585, Japan 2Insect Biomedical Center, Kyoto Institute of Technology, Sakyo-ku, Kyoto 606-8585, Japan.)
Abstract	Previous reports have indicated that the Bombyx mori nucleopolyhedrovirus (BmNPV) nucleic acid binding proteins BRO-B and BRO-E are expressed during the early stage of infection and that the BRO family likely supports the regulation of mRNA; however, no study has directly examined the function of BRO family proteins in virus-permissive cells. Here, we show that BRO-B and BRO-E associate with cellular T-cell intracellular antigen 1 homologue (BmTRN-1), a translational regulator, and other cellular translation-related proteins in silkworm cells during viral infection. We created BM-N cells that expressed BRO-B/E to study molecular interactions between BmTRN-1 and BRO-B/E and how they influenced protein synthesis. Fluorescent microscopy revealed that BmTRN-1 was localized in cytoplasmic foci during BmNPV infection. Immunofluorescence studies confirmed that BmTRN-1 and BRO-B/E were colocalized in the amorphous conspicuous cytoplasmic foci. Reporter gene studies revealed that co-expression of BRO-B/E synergistically led to a significant decrease in protein synthesis from a designed transcript carrying the 5'untranslated region of a cellular mRNA with no significant change of transcript abundance. Additionally, RNA interference-mediated knockdown of BmTRN-1 resulted in a marked inhibition of the ability of BRO-B/E to regulate the transcript. These results suggested that the association of BmTRN-1 with BRO-B/E is responsible for the inhibitory regulation of certain mRNAs at the post-transcriptional level and add an additional mechanism for how baculoviruses control protein synthesis during infection.
File Format	HTM / HTML
ISSN	00221317
Issue Number	Pt 7
Volume Number	96
e-ISSN	14652099
Journal	Journal of General Virology
Language	English
Publisher	Microbiology Society
Publisher Date	2015-07-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Nucleopolyhedrovirus Research Support, Non-u.s. Gov't Protein Biosynthesis Discipline Virology Viral Proteins Metabolism Bombyx Journal Article Virology Rna-binding Proteins Protein Interaction Mapping Microscopy, Confocal Host-pathogen Interactions Animals Gene Expression Regulation, Viral Protein Binding Physiology Microscopy, Fluorescence
Content Type	Text
Resource Type	Article
Subject	Virology

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