NDLI: The predominant species of nonstructural protein 4B in hepatitis C virus-replicating cells is not palmitoylated.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Paul, David Bartenschlager, Ralf McCormick, Christopher
Description	Country affiliation: Germany Author Affiliation: Paul D ( 1Department of Infectious Diseases, Molecular Virology, University of Heidelberg, 69120 Heidelberg, Germany.); Bartenschlager R ( 1Department of Infectious Diseases, Molecular Virology, University of Heidelberg, 69120 Heidelberg, Germany.); McCormick C ( 2Faculty of Medicine and Institute for Life Science, University of Southampton, Southampton SO16 6YD, UK.)
Abstract	Hepatitis C virus (HCV) represents a significant global health burden. Viral replication is thought to occur in close association with remodelled host cell membranes, with non-structural protein 4B (NS4B) being a key player in this process. NS4B is a poorly characterized integral membrane protein, which has been reported to be palmitoylated at its carboxy-terminal end. In order to extend this observation and to establish a functional role for NS4B palmitoylation, we sought to determine the status of this post-translational modification when the protein was expressed as part of a functional viral replicase. We performed direct metabolic labelling and polyethylene glycol-maleimide palmitoylation reporter assays on NS4B expressed in cells containing subgenomic replicons and infectious viral RNA. In a vaccinia virus-based expression system NS4B palmitoylation was detected in a genotype-dependent manner. However, in spite of the high sensitivity of the methods used, no NS4B palmitoylation was found in physiologically more relevant systems. Thus, NS4B palmitoylation is most likely dispensable for HCV RNA replication.
File Format	HTM / HTML
ISSN	00221317
Issue Number	Pt 7
Volume Number	96
e-ISSN	14652099
Journal	Journal of General Virology
Language	English
Publisher	Microbiology Society
Publisher Date	2015-07-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Cell Line Research Support, Non-u.s. Gov't Gene Expression Viral Nonstructural Proteins Lipoylation Discipline Virology Humans Recombinant Proteins Metabolism Journal Article Virology Chemistry Hepatocytes Vaccinia Virus Protein Processing, Post-translational Genetics Physiology Genetic Vectors Hepacivirus
Content Type	Text
Resource Type	Article
Subject	Virology

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