| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Rozila, Ismail Azari, Pedram Munirah, Sha'ban Wan Safwani, Wan Kamarul Zaman Gan, Seng Neon Nur Azurah, Abdul Ghani Jahendran, Jeevanan Pingguan-Murphy, Belinda Chua, Kien Hui |
| Description |
Country affiliation: Malaysia Author Affiliation: Rozila I ( Department of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia Medical Centre, Cheras, Kuala Lumpur, Malaysia.); Azari P ( Department of Chemistry, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.); Munirah S ( Department of Biomedical Science, Kulliyyah of Allied Health Sciences, International Islamic University Malaysia, Kuantan, Pahang, Malaysia.); Wan Safwani WK ( Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur, Malaysia.); Gan SN ( Department of Chemistry, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.); Nur Azurah AG ( Department of Obstetrics and Gynaecology, Universiti Kebangsaan Malaysia Medical Centre, Cheras, Kuala Lumpur, Malaysia.); Jahendran J ( Pantai Cheras Medical Centre, Kuala Lumpur, Malaysia.); Pingguan-Murphy B ( Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur, Malaysia.); Chua KH ( Department of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia Medical Centre, Cheras, Kuala Lumpur, Malaysia.) |
| Abstract |
The osteogenic potential of human adipose-derived stem cells (HADSCs) co-cultured with human osteoblasts (HOBs) using selected HADSCs/HOBs ratios of 1:1, 2:1, and 1:2, respectively, is evaluated. The HADSCs/HOBs were seeded on electrospun three-dimensional poly[(R)-3-hydroxybutyric acid] (PHB) blended with bovine-derived hydroxyapatite (BHA). Monocultures of HADSCs and HOBs were used as control groups. The effects of PHB-BHA scaffold on cell proliferation and cell morphology were assessed by AlamarBlue assay and field emission scanning electron microscopy. Cell differentiation, cell mineralization, and osteogenic-related gene expression of co-culture HADSCs/HOBs were examined by alkaline phosphatase (ALP) assay, alizarin Red S assay, and quantitative real time PCR, respectively. The results showed that co-culture of HADSCs/HOBs, 1:1 grown into PHB-BHA promoted better cell adhesion, displayed a significant higher cell proliferation, higher production of ALP, extracellular mineralization and osteogenic-related gene expression of run-related transcription factor, bone sialoprotein, osteopontin, and osteocalcin compared to other co-culture groups. This result also suggests that the use of electrospun PHB-BHA in a co-culture HADSCs/HOBs system may serve as promising approach to facilitate osteogenic differentiation activity of HADSCs through direct cell-to-cell contact with HOBs. |
| File Format |
HTM / HTML |
| ISSN |
15493296 |
| Issue Number |
2 |
| Volume Number |
104 |
| e-ISSN |
15524965 |
| Journal |
Journal of Biomedical Materials Research Part A |
| Language |
English |
| Publisher |
Wiley |
| Publisher Date |
2016-02-01 |
| Publisher Place |
United States |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Antigens, Differentiation Coculture Techniques Humans Cytology Male Biosynthesis Metabolism Osteoblasts Journal Article Hydroxybutyrates Discipline Biomedical Engineering Stem Cells Tissue Scaffolds Chemistry Animals Cattle Polyesters Female Adipose Tissue Osteogenesis |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Ceramics and Composites Metals and Alloys Biomaterials Biomedical Engineering |
