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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Arndt, Torsten Beyreiß, Reinhild Schröfel, Stefanie Stemmerich, Karsten |
| Description | Author Affiliation: Arndt T ( Bioscientia Institut für Medizinische Diagnostik GmbH, Zentrum für Toxikologie und Forensik, Konrad-Adenauer-Straße 17, Ingelheim D-55218, Germany. Electronic address: torsten.arndt@bioscientia.de.); Beyreiß R ( Bioscientia Institut für Medizinische Diagnostik GmbH, Zentrum für Toxikologie und Forensik, Konrad-Adenauer-Straße 17, Ingelheim D-55218, Germany.); Schröfel S ( Bioscientia Institut für Medizinische Diagnostik GmbH, Zentrum für Toxikologie und Forensik, Konrad-Adenauer-Straße 17, Ingelheim D-55218, Germany.); Stemmerich K ( Bioscientia Institut für Medizinische Diagnostik GmbH, Zentrum für Toxikologie und Forensik, Konrad-Adenauer-Straße 17, Ingelheim D-55218, Germany.) |
| Abstract | BACKGROUND: Ethyl glucuronide (EtG) in urine is considered a marker of recent alcohol consumption. Using immunoassays for EtG screening without confirmatory analysis bears a risk of getting false-positives as shown for trichloroethyl glucuronide from chloral hydrate medication and 1-propyl glucuronide from propanol-based hand disinfection. The aim of the study was to check whether glucuronides of frequently used aliphatic short chain alcohols aside from EtG and 1-propyl glucuronide can cross-react with the DRI(®) Ethyl Glucuronide Assay. METHODS: Aliquots of EtG-free urine were individually spiked with methyl ß-D-glucuronide, 1-propyl ß-D-glucuronide, 2-propyl ß-D-glucuronide, 1-butyl ß-D-glucuronide, 2-butyl ß-D-glucuronide, and tert-butyl ß-D-glucuronide. To check the response rate of the DRI(®) Ethyl Glucuronide Assay to its target analyte, EtG was also added to a native EtG-free urine sample. The spiked alcohol glucuronide concentrations (seven levels up to 10mg/L) and the DRI(®) Ethyl Glucuronide Assay results were evaluated by Passing-Bablok regression analysis. The 95% confidence interval ranges for the slope of the regression function were considered a measure of cross-reaction of the individual alcohol glucuronides with the enzyme immunoassay. RESULTS: 2-Propyl glucuronide showed a cross-reactivity of 69-84% at the 95% probability level, methyl glucuronide, 1-propyl glucuronide, and 1- and 2-butyl glucuronide of 4-9%, and tert-butyl glucuronide almost no cross-reactivity. The response rate for EtG was 87-94% at the 95% probability level. CONCLUSION: The DRI(®) Ethyl Glucuronide Assay shows cross-reaction rates with aliphatic short chain alcohol glucuronides aside from EtG which bear a risk of getting false-positives regarding ethanol consumption. Mass spectrometric detection of EtG is mandatory for confirmation of positive immunological EtG screenings. |
| File Format | HTM / HTML |
| ISSN | 03790738 |
| Volume Number | 241 |
| e-ISSN | 18726283 |
| Journal | Forensic Science International |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-08-01 |
| Publisher Place | Ireland |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Urine Discipline Forensic Science Regression Analysis Chemistry Biological Markers Humans Glucuronides Forensic Toxicology Alcohol Drinking Glucuronates Immunoenzyme Techniques Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Law Pathology and Forensic Medicine |
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