NDLI: Use of dried plasma spots for the quantification of iothalamate in clinical studies.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Hagan, Andrew S. Jones, David R. Agarwal, Rajiv
Description	Country affiliation: United States Author Affiliation: Hagan AS ( Indiana University School of Medicine and Richard L. Roudebush Veterans Administration Medical Center, Indianapolis, Indiana 46202, USA.)
Abstract	BACKGROUND AND OBJECTIVES: Although iothalamate clearances have been widely used to measure GFR, the need for transportation of plasma samples under refrigerated conditions obviates its use in resource-poor situations. Spots of blood or plasma dried on filter paper may provide a solution. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS: Using a validated HPLC technique, iothalamate in dried blood spots of different hematocrits was measured. GFR was measured over 5 hours in 10 subjects with CKD using dried plasma spots and standard methods. RESULTS: Lower hematocrit produced greater area of blood spreading and lowered the recovery of iothalamate from dried blood spots. However, the relationship between iothalamate concentrations in dried plasma spots and plasma showed a regression slope of 0.95 (95% confidence interval=0.92-0.98, P<0.001). Bland-Altman plot of paired sample points (n=116) showed a bias of -4 µg/ml and limits of agreement of -38 to +30 µg/ml. The relationship between GFRs using dried plasma spots and plasma methods also showed an excellent relationship (slope of 0.95, 95% confidence interval=0.82-1.17). Bland-Altman plot of paired GFRs showed a bias of 2 ml/min, with limits of agreement of -6 to +10 ml/min. Precision was generally between 5% and 10%, and accuracy was within 5%. CONCLUSIONS: Although dried blood spots are unsuitable for studies among those patients with very low hematocrit, dried plasma spots correct for this limitation, and this small pilot study shows that it is a reasonably reliable method for quantifying iothalamate and subsequently, determining GFR.
File Format	HTM / HTML
ISSN	15559041
e-ISSN	1555905X
DOI	10.2215/CJN.10471012
Journal	Clinical Journal of the American Society of Nephrology
Issue Number	6
Volume Number	8
Language	English
Publisher	American Society of Nephrology
Publisher Date	2013-06-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Research Support, N.i.h., Extramural Iothalamic Acid Biological Markers Dried Blood Spot Testing Hematocrit Chromatography, High Pressure Liquid Predictive Value Of Tests Kidney Blood Time Factors Validation Studies Diagnosis Renal Insufficiency, Chronic Glomerular Filtration Rate Discipline Nephrology Spectrophotometry, Ultraviolet Physiopathology Reproducibility Of Results Pilot Projects
Content Type	Text
Resource Type	Article
Subject	Transplantation Critical Care and Intensive Care Medicine Nephrology Epidemiology

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