NDLI: Synthesis of the light/pH responsive polymer for immobilization of -amylase.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Yang, Long Lei, Ming Zhao, Min Yang, Hong Zhang, Hong Li, Yan Zhang, Kehu Lei, Zhongli
Description	Country affiliation: China Author Affiliation: Yang L ( Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710119, China.); Lei M ( School of Material Science and Engineering, Shaanxi Normal University, Xi'an 710119, China.); Zhao M ( Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710119, China.); Yang H ( Basic Experimental Teaching Center, Shaanxi Normal University, Xi'an 710062, China.); Zhang H ( Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710119, China.); Li Y ( Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710119, China.); Zhang K ( Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710119, China.); Lei Z ( Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710119, China. Electronic address: lzl2016@snnu.edu.cn.)
Abstract	In this study, light/pH responsive methoxy poly (ethylene glycol)-(5-propargylether-2-nitrobenzyl bromoisobutyrate)-poly methylacrylic acid-b-polystyrene (mPEG-ONB-PMAA-b-PS) polymers were synthesized, and successfully utilized to fabricate micelles and immobilize -amylase. The critical micelle concentrations (CMC) of the polymers were measured with Pyrene Fluorescent Probe Technique. The morphology and diameter of micelles were characterized by transmission electron microscopy (TEM) and dynamic light scattering (DLS). In addition, the effects of pH, temperature and light-responsive on the catalytic activity were investigated. The optimized fabrication conditions of -amylase-loaded micelles which -amylase gave the higher activity were as follows: Immobilization time, 60min; Immobilization temperature, 50°C; enzyme concentration, 10UmL ; PBS buffer, pH=5.4. -Amylase immobilized in these micelles was much more stable than that free -amylase.
File Format	HTM / HTML
ISSN	09284931
Journal	Materials Science and Engineering: C
Volume Number	71
e-ISSN	18730191
Language	English
Publisher	Elsevier
Publisher Date	2017-02-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Content Type	Text
Resource Type	Article
Subject	Mechanics of Materials Biomaterials Condensed Matter Physics Bioengineering Mechanical Engineering

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