NDLI: A novel chitosan hydrogel membrane by an improved electrophoretic deposition and its characteristics in vitro and in vivo.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Li, Wen-Wen Wang, Hai-Yan Zhang, Yu-Qing
Description	Author Affiliation: Li WW ( Silk Biotechnology Laboratory, School of Biology and Basic Medical Sciences, Soochow University, National Engineering Laboratory for Modern Silk, Soochow University, RM702-2303, No. 199, Renai Road, Dushuhu Higher Edu. Town, Suzhou 215123, PR China.); Wang HY ( Silk Biotechnology Laboratory, School of Biology and Basic Medical Sciences, Soochow University, National Engineering Laboratory for Modern Silk, Soochow University, RM702-2303, No. 199, Renai Road, Dushuhu Higher Edu. Town, Suzhou 215123, PR China.); Zhang YQ ( Silk Biotechnology Laboratory, School of Biology and Basic Medical Sciences, Soochow University, National Engineering Laboratory for Modern Silk, Soochow University, RM702-2303, No. 199, Renai Road, Dushuhu Higher Edu. Town, Suzhou 215123, PR China. Electronic address: sericult@suda.edu.cn.)
Abstract	Here, we report a novel chitosan hydrogel membrane (CHM) created by an improved electrophoretic deposition. Unlike a traditional CHM by electrophoretic deposition, the CHM was formed on a nanoporous film as a barrier using a homemade device at a high DC voltage (60 V ). The CHM maximum recovery of 81.7% could be achieved after 1h of electrophoretic deposition. The transparent CHM with an elongation of 42.46% and swelling index of 538.86% was a mixture of type I and type II crystal structures. SEM revealed that the CHM had an irregular net structure. The CHM was sufficient for L-929 mouse fibroblast cell adhesion and growth. To demonstrate immunocompatibility with host tissues, H&E and TGF-ß were observed and the expressions of TNF- and NF-κB were measured up to 3weeks post-implantation. Although these scaffolds demonstrated an initial pro-inflammatory response, the amount of inflammatory cells and the expressions of TGF-ß returned to baseline control values at 3weeks. The expressions of TNF- and NF-κB had no significant difference between the experimental and control groups. Animal experiments showed that the CHM did not incite serious inflammatory reactions. Thus, the CHM is a promising medical biomaterial candidate for loading appropriate cells for use as artificial skin or in transplantation.
File Format	HTM / HTML
ISSN	09284931
Journal	Materials Science and Engineering: C
Volume Number	74
e-ISSN	18730191
Language	English
Publisher	Elsevier
Publisher Date	2017-05-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Content Type	Text
Resource Type	Article
Subject	Mechanics of Materials Biomaterials Condensed Matter Physics Bioengineering Mechanical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in