NDLI: Human genomic DNA isolation from whole blood using a simple microfluidic system with silica- and polymer-based stationary phases.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Günal, Gülçin Kip, Çigdem Ögüt, Sevim Eda Usta, Duygu Deniz Senlik, Erhan Kibar, Günes Tuncel, Ali
Description	Country affiliation: Turkey Author Affiliation: Günal G ( Hacettepe University, Bioengineering Division, Ankara, Turkey.); Kip Ç ( Hacettepe University, Chemical Engineering Department, Ankara, Turkey.); Ögüt SE ( Hacettepe University, Bioengineering Division, Ankara, Turkey.); Usta DD ( Gazi University, Division of Medical Biology & Genetics, Ankara, Turkey.); Senlik E ( Hacettepe University, Division of Nanotechnology and Nanomedicine, Ankara, Turkey.); Kibar G ( Hacettepe University, Bioengineering Division, Ankara, Turkey.); Tuncel A ( Hacettepe University, Bioengineering Division, Ankara, Turkey)
Abstract	Monodisperse-porous silica microspheres 5.1µm in size with a bimodal pore-size distribution (including both mesoporous and macroporous compartments) were obtained using a newly developed staged-shape templated hydrolysis and condensation protocol. Synthesized silica microspheres and monodisperse-porous polymer-based microspheres with different functionalities, synthesized by staged-shape template polymerization, were comparatively tested as sorbents for human genomic DNA (hgDNA) isolation in a microfluidic system. Microcolumns with a permeability range of 1.8-8.5×10 m were fabricated by the slurry-packing of silica- or polymer-based microspheres. The monodisperse-porous silica microspheres showed the best performance in hgDNA isolation in an aqueous buffer medium; >2500ng of hgDNA was recovered with an isolation yield of about 50%, using an hgDNA feed concentration of 100ng/µL. Monodisperse-porous silica microspheres were also evaluated as a sorbent for genomic DNA isolation from human whole blood in the microfluidic system; 14ng of hgDNA was obtained from 10µL of whole blood lysate with an isolation yield of 64%. Based on these results, we conclude that monodisperse-porous silica microspheres with a bimodal pore size distribution are a promising sorbent for the isolation of hgDNA in larger amounts and with higher yields compared to the sorbents previously tried in similar microfluidic systems.
File Format	HTM / HTML
ISSN	09284931
Journal	Materials Science and Engineering: C
Volume Number	74
e-ISSN	18730191
Language	English
Publisher	Elsevier
Publisher Date	2017-05-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Content Type	Text
Resource Type	Article
Subject	Mechanics of Materials Biomaterials Condensed Matter Physics Bioengineering Mechanical Engineering

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