| Author |
Sandoval, Juan Díaz-Lagares, Angel Salgado, Rocío Servitje, Octavio Climent, Fina Ortiz-Romero, Pablo L. Pérez-Ferriols, Amparo Garcia-Muret, Maria P. Estrach, Teresa Garcia, Mar Nonell, Lara Esteller, Manel Pujol, Ramon M. Espinet, Blanca Gallardo, Fernando |
| Description |
Country affiliation: Spain Author Affiliation: Sandoval J ( Cancer Epigenetics and Biology Program (PEBC), Bellvitge Biomedical Research Institute (IDIBELL), L'Hospitalet, Barcelona, Spain.); Díaz-Lagares A ( Cancer Epigenetics and Biology Program (PEBC), Bellvitge Biomedical Research Institute (IDIBELL), L'Hospitalet, Barcelona, Spain.); Salgado R ( Laboratori de Citogenètica Molecular, Servei de Patologia, Hospital del Mar, Barcelona, Spain.); Servitje O ( Servei de Dermatologia i Patologia, Hospital Universitari de Bellvitge, L'Hospitalet de Llobregat, Barcelona, Spain.); Climent F ( Servei de Dermatologia i Patologia, Hospital Universitari de Bellvitge, L'Hospitalet de Llobregat, Barcelona, Spain.); Ortiz-Romero PL ( Servicio de Dermatologia, Hospital 12 de Octubre, University Complutense Madrid, Madrid, Spain.); Pérez-Ferriols A ( Servicio de Dermatologia, Hospital General de Valencia, Valencia, Spain.); Garcia-Muret MP ( Servei de Dermatologia, Hospital de Sant Pau, Barcelona, Spain.); Estrach T ( Servei de Dermatologia, Hospital Clínic-IDIBAPS, Universitat de Barcelona, Barcelona, Spain.); Garcia M ( Laboratori de Citogenètica Molecular, Servei de Patologia, Hospital del Mar, Barcelona, Spain.); Nonell L ( Servei d'Anàlisi de Microarrays, IMIM (Hospital del Mar Medical Research Institute), Barcelona, Spain.); Esteller M ( 1] Cancer Epigenetics and Biology Program (PEBC), Bellvitge Biomedical Research Institute (IDIBELL), L'Hospitalet, Barcelona, Spain [2] Department of Physiological Science II, School of Medicine, University of Barcelona, Barcelona, Spain [3] Institució Catalana de Recerca i Estudis Avançats (ICREA),); Pujol RM ( Servei de Dermatologia, Hospital del Mar, Universitat Autònoma de Barcelona, Barcelona, Spain.); Espinet B ( 1] Laboratori de Citogenètica Molecular, Servei de Patologia, Hospital del Mar, Barcelona, Spain [2] Cancer Research Program, IMIM (Hospital del Mar Medical Research Institute), Barcelona, Spain.); Gallardo F ( 1] Servei de Dermatologia, Hospital del Mar, Universitat Autònoma de Barcelona, Barcelona, Spain [2] Cancer Research Program, IMIM (Hospital del Mar Medical Research Institute), Barcelona, Spain.) |
| Abstract |
MicroRNAs usually regulate gene expression negatively, and aberrant expression has been involved in the development of several types of cancers. Microarray profiling of microRNA expression was performed to define a microRNA signature in a series of mycosis fungoides tumor stage (MFt, n=21) and CD30+ primary cutaneous anaplastic large cell lymphoma (CD30+ cALCL, n=11) samples in comparison with inflammatory dermatoses (ID, n=5). Supervised clustering confirmed a distinctive microRNA profile for cutaneous T-cell lymphoma (CTCL) with respect to ID. A 40 microRNA signature was found in MFt including upregulated onco-microRNAs (miR-146a, miR-142-3p/5p, miR-21, miR-181a/b, and miR-155) and downregulated tumor-suppressor microRNAs (miR-200ab/429 cluster, miR-10b, miR-193b, miR-141/200c, and miR-23b/27b). Regarding CD30+ cALCL, 39 differentially expressed microRNAs were identified. Particularly, overexpression of miR-155, miR-21, or miR-142-3p/5p and downregulation of the miR-141/200c clusters were observed. DNA methylation in microRNA gene promoters, as expression regulatory mechanism for deregulated microRNAs, was analyzed using Infinium 450K array and approximately one-third of the differentially expressed microRNAs showed significant DNA methylation differences. Two different microRNA methylation signatures for MFt and CD30+ cALCL were found. Correlation analysis showed an inverse relationship for microRNA promoter methylation and microRNA expression. These results reveal a subgroup-specific epigenetically regulated microRNA signatures for MFt and CD30+ cALCL patients. |
