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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Taylor, Steven W. Clarke, Nigel J. Chen, Zhaohui McPhaul, Michael J. |
| Description | Country affiliation: United States Author Affiliation: Taylor SW ( Quest Diagnostics Nichols Institute, San Juan Capistrano, CA, United States.); Clarke NJ ( Quest Diagnostics Nichols Institute, San Juan Capistrano, CA, United States. Electronic address: Nigel.J.Clarke@questdiagnostics.com.); Chen Z ( Quest Diagnostics Nichols Institute, San Juan Capistrano, CA, United States.); McPhaul MJ ( Quest Diagnostics Nichols Institute, San Juan Capistrano, CA, United States.) |
| Abstract | BACKGROUND: Measurements of fasting levels of insulin and C-peptide are useful in documenting insulin resistance and may help predict development of diabetes mellitus. However, the specific insulin and C-peptide levels associated with specific degrees of insulin resistance have not been defined, owing to marked variability among immunoassays and lack of standardization. Herein, we describe a multiplexed liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay for intact insulin and C-peptide. METHODS: Insulin and C-peptide were enriched from patient sera using monoclonal antibodies immobilized on magnetic beads and processed on a robotic liquid handler. Eluted peptides were analyzed by LC-MS/MS. Bovine insulin and a stable isotopically-labeled (13C/15N) C-peptide were utilized as internal standards. RESULTS: The assay had an analytical measurement range of 3 to 320 µIU/ml (18 to 1920 pmol/l) for insulin and 0.11 to 27.2 ng/ml (36 to 9006 pmol/l) for C-peptide. Intra- and inter-day assay variation was less than 11% for both peptides. Of the 5 insulin analogs commonly prescribed to treat diabetes, only the recombinant drug insulin lispro caused significant interference for the determination of endogenous insulin. There were no observed interferences for C-peptide. CONCLUSION: We developed and validated a high-throughput, quantitative, multiplexed LC-MS/MS assay for intact insulin and C-peptide. |
| File Format | HTM / HTML |
| ISSN | 00098981 |
| Journal | Clinica Chimica Acta |
| Volume Number | 455 |
| e-ISSN | 18733492 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-04-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry (medical) Clinical Biochemistry Biochemistry |
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