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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Roche, Lucie Pinguet, Jérémy Herviou, Pauline Libert, Frédéric Chenaf, Chouki Eschalier, Alain Authier, Nicolas Richard, Damien |
| Description | Author Affiliation: Roche L ( CHU Clermont-Ferrand, Service de Pharmacologie Médicale, Laboratoire de pharmacologie-toxicologie, F-63003 Clermont-Ferrand, France); Pinguet J ( CHU Clermont-Ferrand, Service de Pharmacologie Médicale, Laboratoire de pharmacologie-toxicologie, F-63003 Clermont-Ferrand, France); Herviou P ( CHU Clermont-Ferrand, Service de Pharmacologie Médicale, Laboratoire de pharmacologie-toxicologie, F-63003 Clermont-Ferrand, France); Libert F ( CHU Clermont-Ferrand, Service de Pharmacologie Médicale, Laboratoire de pharmacologie-toxicologie, F-63003 Clermont-Ferrand, France); Chenaf C ( CHU Clermont-Ferrand, Service de Pharmacologie Médicale, Laboratoire de pharmacologie-toxicologie, F-63003 Clermont-Ferrand, France); Eschalier A ( CHU Clermont-Ferrand, Service de Pharmacologie Médicale, Laboratoire de pharmacologie-toxicologie, F-63003 Clermont-Ferrand, France); Authier N ( CHU Clermont-Ferrand, Service de Pharmacologie Médicale, Laboratoire de pharmacologie-toxicologie, F-63003 Clermont-Ferrand, France); Richard D ( CHU Clermont-Ferrand, Service de Pharmacologie Médicale, Laboratoire de pharmacologie-toxicologie, F-63003 Clermont-Ferrand, France) |
| Abstract | BACKGROUND: In clinical and forensic toxicology, fast and specific methods are needed for the screening of different classes of drugs. A complete general unknown screening procedure was developed using turbulent flow chromatography with electrospray ionization and Orbitrap mass spectrometry. METHODS: After protein precipitation, samples were injected directly into the turbulent flow chromatographic system and analyzed with an Orbitrap mass spectrometer. The Exactive® operated in positive and negative modes with alternated high collision dissociation in order to obtain characteristic fragments. We built a library containing 616 compounds by analyzing a reference standard for all the molecules. RESULTS: Identification was based on retention time, accurate measured mass, isotopic pattern and presence of specific fragments. For each substance, we set a calibration range encompassing infra-therapeutic, therapeutic, supra-therapeutic and toxic concentrations in order to generate semi-quantitative result. For 65% of the components, the limit of detection was below 5 ng/mL. The validation process showed the approach to be selective, sensitive, accurate and precise. CONCLUSION: The method has been accredited by COFRAC (French Accreditation Committee) according to the ISO 15189 standard. Applicability was successfully tested by analyzing authentic serum, urine and whole blood samples. |
| File Format | HTM / HTML |
| ISSN | 00098981 |
| Journal | Clinica Chimica Acta |
| Volume Number | 455 |
| e-ISSN | 18733492 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-04-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry (medical) Clinical Biochemistry Biochemistry |
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