NDLI: Study of phase transformation of guanosine 5'-monophosphate in drowning-out crystallization.

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Study of phase transformation of guanosine 5'-monophosphate in drowning-out crystallization.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kang, Jeongki Tuan, Nguyen Anh Kim, Jong-Min Chang, Sang-Mok Kim, Woo-Sik
Description	Author Affiliation: Kang J ( Department of Chemical Engineering, Kyunghee University, Kyungki-do, Korea.)
Abstract	The present study used a mechanistic approach to control the phase transformation of guanosine 5'-monophosphate (GMP) via the operating conditions of agitation and feed concentration during drowning-out crystallization. First, Fourier transform infrared and UV/vis spectrophotometry were successfully applied to monitor the mass fraction of GMP polymorphs (amorphous and hydrate crystalline GMPs) and GMP supersaturation, respectively, during the crystallization. The phase transformation of amorphous GMP into hydrate crystals was significantly influenced by the agitation, which promoted the mass transfer of GMP dissolution and growth. Therefore, the phase transformation was quickly finished when increasing the agitation speed. However, a high agitation caused breakage of the hydrate crystals, resulting in a reduced crystal size with a bimodal distribution. The phase transformation was also influenced by the GMP feed concentration, as the crystal growth was promoted and the crystal size increased when increasing the feed concentration up to 61 g/l. However, a further increase in the feed concentration caused secondary nucleation due to the induction of a high supersaturation level during the phase transformation, leading to a small crystal size with a bimodal distribution. In addition, the rectangular-shaped hydrate GMP crystals exhibited a higher growth rate in the b direction rather than the a direction. Therefore, the crystal morphology shifted from a long rectangle to a square when increasing the feed concentration.
File Format	HTM / HTML
ISSN	02732289
Issue Number	2
Journal	Applied Biochemistry and Biotechnology
Volume Number	160
e-ISSN	15590291
Language	English
Publisher	Springer
Publisher Date	2010-01-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Biochemistry Discipline Biotechnology Guanosine Monophosphate Chemistry Thionucleotides Crystallization Phase Transition Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

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