NDLI: Protein-Coated Microcrystals of Pseudomonas aeruginosa PseA lipase

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Protein-Coated Microcrystals of Pseudomonas aeruginosa PseA lipase

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Protein-Coated Microcrystals of Pseudomonas aeruginosa PseA lipase

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Gaur, Ruchi Gupta, G. N. Vamsikrishnan, M. Khare, S. K.
Description	Country affiliation: India Author Affiliation: Gaur R ( Chemistry Department, Indian Institute of Technology, Hauz Khas, New Delhi, India.)
Abstract	Highly active Pseudomonas aeruginosa lipase protein-coated microcrystals (PAL PCMC) have been prepared by immobilization of protein onto $K_{2}SO_{4}$ as excipient solid support carrier and n-propanol as precipitating solvent. Transmission electron micrographs confirmed the formation of PAL PCMC. These PCMC were found to be a catalytically more active and stable preparation for p-nitrophenyl palmitate hydrolysis in n-heptane, compared to free lipase. The $V_{max},$ $K_{m},$ and temperature optimum for PAL PCMC increased from 0.49 to 5.66 nmol $min^{−1}$ $mg^{−1},$ 589 to 679.8 mmol, and 40°C to 45°C, respectively. These were thermally more stable with 4.65, 2.56, and 1.24-fold improvement in half lives at 45°C, 55°C, and 60°C compared to free P. aeruginosa PseA lipase. Their catalytic efficiency was enhanced by tenfold over that of free enzyme. PAL PCMC offer a simple and effective technique for obtaining stable and efficient lipase preparation for biocatalysis in nonaqueous medium.
File Format	HTM / HTML
ISSN	02732289
Issue Number	2-3
Volume Number	151
e-ISSN	15590291
Journal	Applied Biochemistry and Biotechnology
Language	English
Publisher	Springer
Publisher Date	2008-12-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Biochemistry Discipline Biotechnology Enzymes, Immobilized Chemistry Lipase Crystallization Enzyme Stability Kinetics Microscopy, Electron, Transmission Pseudomonas Aeruginosa Enzymology Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

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